Restriction map for
pFN21AE9447
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BciVI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
BstAPI CelII ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SfcI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 907 GTATAC GT^[AC][GT]AC
AclI 413 AACGTT AA^CGTT
AlwI 953 GATCC GGATC(4/5)
AocI 306 CCTGAGG CC^T[ACGT]AGG
Asp700I 706 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 6 CTCGGG C^[CT]CG[GA]G
BbeI 72 GGCGCC GGCGC^C
BbsI 928 GTCTTC GAAGAC(2/6)
BcgI 362 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 112 CCCGG CC^[GC]GG
BfaI 556 CTAG C^TAG
BglI 460 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 708 AGATCT A^GATCT
BpmI 462 CTGGAG CTGGAG(16/14)
BpuAI 928 GTCTTC GAAGAC(2/6)
BseRI 232 CTCCTC GAGGAG(10/8)
BsgI 561 CTGCAC GTGCAG(16/14)
BsmI 689 GAATGC GAATGC(1/-1)
BsoBI 6 CTCGGG C^[CT]CG[GA]G
BspHI 932 TCATGA T^CATGA
Bst1107I 907 GTATAC GTA^TAC
BstXI 657 CCATTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 907 GTATAC GTA^TAC
Bsu36I 306 CCTGAGG CC^T[ACGT]AGG
DraI 738 TTTAAA TTT^AAA
Eco81I 306 CCTGAGG CC^T[ACGT]AGG
EcoNI 302 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 581 CCAAGG C^C[AT][AT]GG
HgaI 164 GCGTC GACGC(5/10)
KasI 72 GGCGCC G^GCGCC
KspI 53 CCGCGG CCGC^GG
MaeI 556 CTAG C^TAG
NarI 72 GGCGCC GG^CGCC
NciI 112 CCCGG CC^[GC]GG
PflMI 1015 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 413 AACGTT AA^CGTT
PvuII 1019 CAGCTG CAG^CTG
RcaI 932 TCATGA T^CATGA
SacII 53 CCGCGG CCGC^GG
SexAI 851 ACCTGGT A^CC[AT]GGT
SfiI 459 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 72 GGCGCC GGC^GCC
SgrAI 74 CGCCGGCG C[GA]^CCGG[CT]G
StuI 997 AGGCCT AGG^CCT
StyI 581 CCAAGG C^C[AT][AT]GG
Van91I 1015 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 149 CCATTGGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 706 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 72 GGCGCC G[GA]^CG[CT]C
163 GGCGTC
AluI 965 AGCT AG^CT
1020 AGCT
AlwNI 964 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1016 CAGCAGCTG
AspHI 184 GTGCTC G[AT]GC[AT]^C
509 GAGCAC
AvaII 429 GGTCC G^G[AT]CC
1172 GGTCC
BalI 666 TGGCCA TGG^CCA
758 TGGCCA
BanII 90 GAGCCC G[GA]GC[CT]^C
144 GGGCTC
BsaHI 72 GGCGCC G[GA]^CG[CT]C
163 GGCGTC
BsiHKAI 184 GTGCTC G[AT]GC[AT]^C
509 GAGCAC
BspMI 176 ACCTGC ACCTGC(4/8)
260 ACCTGC
BsrDI 384 CATTGC GCAATG(2/0)
658 CATTGC
BsrFI 69 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
BstBI 686 TTCGAA TT^CGAA
947 TTCGAA
BstEII 282 GGTCACC G^GT[ACGT]ACC
847 GGTCACC
BstPI 282 GGTCACC G^GT[ACGT]ACC
847 GGTCACC
BstYI 708 AGATCT [GA]^GATC[CT]
952 AGATCC
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
75 GCCGGC
EcoO65I 282 GGTCACC G^GT[ACGT]ACC
847 GGTCACC
Hin1I 72 GGCGCC G[GA]^CG[CT]C
163 GGCGTC
HincII 241 GTCAAC GT[CT]^[GA]AC
1147 GTTGAC
HindII 241 GTCAAC GT[CT]^[GA]AC
1147 GTTGAC
HinfI 595 GATTC G^A[ACGT]TC
1135 GAATC
MaeII 414 ACGT A^CGT
545 ACGT
MflI 708 AGATCT [GA]^GATC[CT]
952 AGATCC
MluNI 666 TGGCCA TGG^CCA
758 TGGCCA
MscI 666 TGGCCA TGG^CCA
758 TGGCCA
NaeI 69 GCCGGC GCC^GGC
75 GCCGGC
NgoMIV 69 GCCGGC G^CCGGC
75 GCCGGC
NspV 686 TTCGAA TT^CGAA
947 TTCGAA
SfaNI 586 GATGC GCATC(5/9)
804 GATGC
SfuI 686 TTCGAA TT^CGAA
947 TTCGAA
TfiI 595 GATTC G^A[AT]TC
1135 GAATC
Tsp509I 448 AATT ^AATT
720 AATT
XhoII 708 AGATCT [GA]^GATC[CT]
952 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AfaI BanI BbvCI
BbvI BclI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I