Restriction map for
pFN21AE1848
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI AlwNI AocI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BciVI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeII MamI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1078 GTCTAC GT^[AC][GT]AC
AcsI 541 GAATTC [GA]^AATT[CT]
AcyI 699 GGCGCC G[GA]^CG[CT]C
AflII 514 CTTAAG C^TTAAG
AlwI 567 GGATC GGATC(4/5)
ApoI 541 GAATTC [GA]^AATT[CT]
BalI 410 TGGCCA TGG^CCA
BbeI 699 GGCGCC GGCGC^C
BclI 617 TGATCA T^GATCA
BfaI 887 CTAG C^TAG
BfrI 514 CTTAAG C^TTAAG
BsaHI 699 GGCGCC G[GA]^CG[CT]C
BsaI 634 GAGACC GGTCTC(1/5)
BsrBI 193 GAGCGG CCGCTC(-3/-3)
BssHII 22 GCGCGC G^CGCGC
BssSI 601 CTCGTG CACGAG(-5/-1)
BstAPI 1052 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 567 GGATCT [GA]^GATC[CT]
EcoRI 541 GAATTC G^AATTC
FbaI 617 TGATCA T^GATCA
Hin1I 699 GGCGCC G[GA]^CG[CT]C
HinfI 880 GACTC G^A[ACGT]TC
KasI 699 GGCGCC G^GCGCC
MaeI 887 CTAG C^TAG
MaeIII 1003 GTGAC ^GT[ACGT]AC
MboII 948 GAAGA GAAGA(8/7)
MflI 567 GGATCT [GA]^GATC[CT]
MluNI 410 TGGCCA TGG^CCA
MscI 410 TGGCCA TGG^CCA
MseI 515 TTAA T^TAA
NaeI 781 GCCGGC GCC^GGC
NarI 699 GGCGCC GG^CGCC
NcoI 651 CCATGG C^CATGG
NgoMIV 781 GCCGGC G^CCGGC
NotI 148 GCGGCCGC GC^GGCCGC
NspI 209 GCATGT [GA]CATG^[CT]
PaeR7I 271 CTCGAG C^TCGAG
PflMI 708 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 880 GACTC GAGTC(4/5)
SfoI 699 GGCGCC GGC^GCC
TaqI 272 TCGA T^CGA
Tru9I 515 TTAA T^TAA
Tsp45I 1003 GTGAC ^GT[GC]AC
Tsp509I 542 AATT ^AATT
TthHB8I 272 TCGA T^CGA
Van91I 708 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 271 CTCGAG C^TCGAG
XhoII 567 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 446 GTAC GT^AC
519 GTAC
Aor51HI 299 AGCGCT AGC^GCT
440 AGCGCT
BanII 109 GAGCCC G[GA]GC[CT]^C
575 GAGCCC
BcgI 786 CGAGGCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
524 GCAGCAAGTTCG
BseRI 384 CTCCTC GAGGAG(10/8)
1072 GAGGAG
BsmBI 238 GAGACG CGTCTC(1/5)
742 GAGACG
BsmFI 221 GGGAC GGGAC(10/14)
12 GTCCC
BsrFI 179 ACCGGC [GA]^CCGG[CT]
781 GCCGGC
Cfr10I 179 ACCGGC [GA]^CCGG[CT]
781 GCCGGC
DpnII 568 GATC ^GATC
618 GATC
EagI 80 CGGCCG C^GGCCG
149 CGGCCG
EclXI 80 CGGCCG C^GGCCG
149 CGGCCG
Eco47III 299 AGCGCT AGC^GCT
440 AGCGCT
Eco52I 80 CGGCCG C^GGCCG
149 CGGCCG
EcoT14I 413 CCAAGG C^C[AT][AT]GG
651 CCATGG
HphI 615 GGTGA GGTGA(8/7)
1002 GGTGA
KspI 35 CCGCGG CCGC^GG
975 CCGCGG
MboI 568 GATC ^GATC
618 GATC
NdeII 568 GATC ^GATC
618 GATC
PpuMI 10 GGGTCCC [GA]G^G[AT]CC[CT]
221 GGGACCC
RsaI 446 GTAC GT^AC
519 GTAC
SacII 35 CCGCGG CCGC^GG
975 CCGCGG
Sau3AI 568 GATC ^GATC
618 GATC
SfaNI 723 GCATC GCATC(5/9)
962 GCATC
SmaI 225 CCCGGG CCC^GGG
915 CCCGGG
StuI 170 AGGCCT AGG^CCT
482 AGGCCT
StyI 413 CCAAGG C^C[AT][AT]GG
651 CCATGG
XmaCI 225 CCCGGG C^CCGGG
915 CCCGGG
XmaI 225 CCCGGG C^CCGGG
915 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI Alw44I ApaLI
AspHI AvaI AvaII BanI BbvI
BcnI BglI BmyI BpmI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SmlI TseI TspRI