Restriction map for
pFN21AE2520
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eco47III
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MflI
MluI MroI MslI MvnI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AhdI 1573 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1003 GGGCCC GGGCC^C
AspEI 1573 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1726 GAGCTC G[AT]GC[AT]^C
BalI 1355 TGGCCA TGG^CCA
BanI 69 GGTGCC G^G[CT][GA]CC
BciVI 1224 GGATAC GTATCC(6/5)
BcnI 1034 CCGGG CC^[GC]GG
BglI 1348 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 262 GGTCTC GGTCTC(1/5)
BsgI 968 CTGCAC GTGCAG(16/14)
BsiEI 890 CGGCCG CG[GA][CT]^CG
BsiHKAI 1726 GAGCTC G[AT]GC[AT]^C
BsmFI 924 GGGAC GGGAC(10/14)
BsmI 1248 GAATGC GAATGC(1/-1)
BspMI 381 ACCTGC ACCTGC(4/8)
BsrDI 356 CATTGC GCAATG(2/0)
BsrFI 888 GCCGGC [GA]^CCGG[CT]
BstAPI 25 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 783 GCGC GCG^C
Cfr10I 888 GCCGGC [GA]^CCGG[CT]
DrdI 163 GACCATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1293 CCGTGG C^C[GA][CT]GG
EagI 890 CGGCCG C^GGCCG
Eam1105I 1573 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 890 CGGCCG C^GGCCG
Eco52I 890 CGGCCG C^GGCCG
EcoNI 994 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 313 ATGCAT ATGCA^T
FauI 471 GCGGG CCCGC(4/6)
HhaI 783 GCGC GCG^C
HinP1I 783 GCGC G^CGC
MfeI 1184 CAATTG C^AATTG
MluNI 1355 TGGCCA TGG^CCA
MscI 1355 TGGCCA TGG^CCA
MunI 1184 CAATTG C^AATTG
NaeI 888 GCCGGC GCC^GGC
NciI 1034 CCGGG CC^[GC]GG
NgoMIV 888 GCCGGC G^CCGGC
NsiI 313 ATGCAT ATGCA^T
NspI 315 GCATGC [GA]CATG^[CT]
PflMI 73 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1579 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 1726 GAGCTC GAGCT^C
SapI 1723 GAAGAGC GCTCTTC(1/4)
SmlI 386 CTCAAG C^T[CT][GA]AG
SphI 315 GCATGC GCATG^C
StuI 1448 AGGCCT AGG^CCT
Van91I 73 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 333 AAATTC [GA]^AATT[CT]
422 AAATTC
AlwI 666 GGATC GGATC(4/5)
1384 GGATC
ApoI 333 AAATTC [GA]^AATT[CT]
422 AAATTC
BanII 1003 GGGCCC G[GA]GC[CT]^C
1726 GAGCTC
BfaI 248 CTAG C^TAG
362 CTAG
DpnII 667 GATC ^GATC
1385 GATC
EaeI 890 CGGCCG [CT]^GGCC[GA]
1355 TGGCCA
EcoT14I 1486 CCTTGG C^C[AT][AT]GG
1549 CCAAGG
MaeI 248 CTAG C^TAG
362 CTAG
MboI 667 GATC ^GATC
1385 GATC
NdeII 667 GATC ^GATC
1385 GATC
Sau3AI 667 GATC ^GATC
1385 GATC
StyI 1486 CCTTGG C^C[AT][AT]GG
1549 CCAAGG
Tsp45I 1212 GTCAC ^GT[GC]AC
1521 GTCAC
TspRI 235 CAGTG CA[GC]TG[ACGT][ACGT]^
1629 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboII MnlI
MseI MspA1I MspI MvaI MwoI
NlaIII NlaIV PleI PvuII RsaI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp509I