Restriction map for
pFN21AE1903
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BpuAI BsaBI BsaHI BsaI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspHI BspMI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MamI MluI MslI MspA1I MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 493 GAATTC [GA]^AATT[CT]
AgeI 755 ACCGGT A^CCGGT
Aor51HI 29 AGCGCT AGC^GCT
ApoI 493 GAATTC [GA]^AATT[CT]
Asp700I 365 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 877 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 42 CCCGGG C^[CT]CG[GA]G
AvaII 656 GGACC G^G[AT]CC
BalI 338 TGGCCA TGG^CCA
BglI 14 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 956 AGATCT A^GATCT
BsaAI 121 TACGTG [CT]AC^GT[GA]
BseRI 700 GAGGAG GAGGAG(10/8)
BsoBI 42 CCCGGG C^[CT]CG[GA]G
BspLU11I 1013 ACATGT A^CATGT
BsrBI 139 GAGCGG CCGCTC(-3/-3)
BsrFI 755 ACCGGT [GA]^CCGG[CT]
BstAPI 146 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 956 AGATCT [GA]^GATC[CT]
CfoI 30 GCGC GCG^C
Cfr10I 755 ACCGGT [GA]^CCGG[CT]
EaeI 338 TGGCCA [CT]^GGCC[GA]
EarI 907 GAAGAG CTCTTC(1/4)
Eco47III 29 AGCGCT AGC^GCT
EcoNI 1212 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 493 GAATTC G^AATTC
HaeII 29 AGCGCT [GA]GCGC^[CT]
HhaI 30 GCGC GCG^C
HinP1I 30 GCGC G^CGC
HpaI 535 GTTAAC GTT^AAC
HphI 167 TCACC GGTGA(8/7)
Ksp632I 907 GAAGAG CTCTTC(1/4)
MfeI 554 CAATTG C^AATTG
MflI 956 AGATCT [GA]^GATC[CT]
MluNI 338 TGGCCA TGG^CCA
MscI 338 TGGCCA TGG^CCA
MunI 554 CAATTG C^AATTG
PflFI 877 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1032 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 755 ACCGGT A^CCGGT
PleI 1169 GACTC GAGTC(4/5)
PpuMI 655 GGGACCC [GA]G^G[AT]CC[CT]
SfiI 13 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 42 CCCGGG CCC^GGG
TaqI 491 TCGA T^CGA
TspRI 151 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 877 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 491 TCGA T^CGA
Van91I 1032 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 956 AGATCT [GA]^GATC[CT]
XmaCI 42 CCCGGG C^CCGGG
XmaI 42 CCCGGG C^CCGGG
XmnI 365 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 836 TCCGGA T^CCGGA
914 TCCGGA
AflIII 1007 ACGTGT A^C[GA][CT]GT
1013 ACATGT
AlwI 850 GATCC GGATC(4/5)
583 GGATC
BfaI 941 CTAG C^TAG
1162 CTAG
BlpI 843 GCTCAGC GC^T[ACGT]AGC
966 GCTCAGC
BpmI 25 CTGGAG CTGGAG(16/14)
952 CTGGAG
Bpu1102I 843 GCTCAGC GC^T[ACGT]AGC
966 GCTCAGC
BseAI 836 TCCGGA T^CCGGA
914 TCCGGA
BsmFI 89 GGGAC GGGAC(10/14)
655 GGGAC
BspEI 836 TCCGGA T^CCGGA
914 TCCGGA
BsrDI 1224 CATTGC GCAATG(2/0)
221 GCAATG
BsrI 296 ACTGG ACTGG(1/-1)
1064 ACTGG
BstF5I 870 CATCC GGATG(2/0)
1258 CATCC
CelII 843 GCTCAGC GC^T[ACGT]AGC
966 GCTCAGC
DraII 11 AGGGCCT [GA]G^G[ACGT]CC[CT]
655 GGGACCC
DsaI 275 CCATGG C^C[GA][CT]GG
1037 CCATGG
EcoO109I 11 AGGGCCT [GA]G^G[ACGT]CC[CT]
655 GGGACCC
FauI 63 GCGGG CCCGC(4/6)
141 GCGGG
FokI 870 CATCC GGATG(9/13)
1258 CATCC
HincII 133 GTCAAC GT[CT]^[GA]AC
535 GTTAAC
HindII 133 GTCAAC GT[CT]^[GA]AC
535 GTTAAC
MaeI 941 CTAG C^TAG
1162 CTAG
MroI 836 TCCGGA T^CCGGA
914 TCCGGA
NcoI 275 CCATGG C^CATGG
1037 CCATGG
NspI 1013 ACATGT [GA]CATG^[CT]
1055 ACATGC
PstI 36 CTGCAG CTGCA^G
70 CTGCAG
SacI 964 GAGCTC GAGCT^C
984 GAGCTC
ScaI 158 AGTACT AGT^ACT
519 AGTACT
SfaNI 200 GATGC GCATC(5/9)
486 GCATC
Tsp45I 452 GTGAC ^GT[GC]AC
643 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AspHI BanII
BbvI BcnI BmyI BsaJI BsaWI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
Bsp1407I BsrGI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfcI SspBI
StyI TfiI Tru9I TseI Tsp509I
XcmI