Restriction map for
pFN21AE2512
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# NO CUT ENZYMES ( 150 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsgI BsiEI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KasI KpnI MaeI MamI
MfeI MluI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 386 GACGTC GACGT^C
AccII 832 CGCG CG^CG
AccIII 419 TCCGGA T^CCGGA
AcyI 386 GACGTC G[GA]^CG[CT]C
AfaI 30 GTAC GT^AC
AspHI 355 GAGCTC G[AT]GC[AT]^C
AvaII 291 GGACC G^G[AT]CC
BalI 656 TGGCCA TGG^CCA
BanI 222 GGTGCC G^G[CT][GA]CC
BsaHI 386 GACGTC G[GA]^CG[CT]C
BseAI 419 TCCGGA T^CCGGA
BsiHKAI 355 GAGCTC G[AT]GC[AT]^C
BsmBI 384 GAGACG CGTCTC(1/5)
BspEI 419 TCCGGA T^CCGGA
BspMI 746 ACCTGC ACCTGC(4/8)
BstUI 832 CGCG CG^CG
BstYI 233 AGATCC [GA]^GATC[CT]
DraII 290 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 831 CCGCGG C^C[GA][CT]GG
EaeI 656 TGGCCA [CT]^GGCC[GA]
EcoO109I 290 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 698 CCAAGG C^C[AT][AT]GG
EcoT22I 544 ATGCAT ATGCA^T
Hin1I 386 GACGTC G[GA]^CG[CT]C
HphI 848 TCACC GGTGA(8/7)
KspI 831 CCGCGG CCGC^GG
MaeII 387 ACGT A^CGT
MflI 233 AGATCC [GA]^GATC[CT]
MluNI 656 TGGCCA TGG^CCA
MroI 419 TCCGGA T^CCGGA
MscI 656 TGGCCA TGG^CCA
MslI 667 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 832 CGCG CG^CG
NlaIV 222 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 544 ATGCAT ATGCA^T
PleI 184 GAGTC GAGTC(4/5)
PpuMI 290 AGGACCT [GA]G^G[AT]CC[CT]
PstI 748 CTGCAG CTGCA^G
RsaI 30 GTAC GT^AC
SacI 355 GAGCTC GAGCT^C
SacII 831 CCGCGG CCGC^GG
SapI 64 GAAGAGC GCTCTTC(1/4)
SbfI 747 CCTGCAGG CCTGCA^GG
SfcI 748 CTGCAG C^T[GA][CT]AG
Sse8387I 747 CCTGCAGG CCTGCA^GG
StyI 698 CCAAGG C^C[AT][AT]GG
TfiI 630 GATTC G^A[AT]TC
Tsp45I 847 GTCAC ^GT[GC]AC
XhoII 233 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwNI 101 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
307 CAGGCGCTG
BanII 355 GAGCTC G[GA]GC[CT]^C
467 GGGCTC
BbvCI 502 GCTGAGG CCTCAGC(-5/-2)
684 GCTGAGG
BcnI 515 CCCGG CC^[GC]GG
851 CCGGG
BmyI 355 GAGCTC G[AGT]GC[ACT]^C
467 GGGCTC
BsaI 109 GAGACC GGTCTC(1/5)
118 GAGACC
BsaWI 272 ACCGGA [AT]^CCGG[AT]
419 TCCGGA
Bsp1286I 355 GAGCTC G[AGT]GC[ACT]^C
467 GGGCTC
HaeII 309 GGCGCT [GA]GCGC^[CT]
854 GGCGCT
HinfI 184 GAGTC G^A[ACGT]TC
630 GATTC
MaeIII 557 GTTAC ^GT[ACGT]AC
847 GTCAC
NciI 515 CCCGG CC^[GC]GG
851 CCGGG
PvuII 56 CAGCTG CAG^CTG
682 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsmAI BsrBI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I