Restriction map for
pFN21AE5316
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# NO CUT ENZYMES ( 148 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsgI BsiEI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KasI
KpnI MaeI MamI MfeI MluI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 386 GACGTC GACGT^C
AccII 823 CGCG CG^CG
AccIII 419 TCCGGA T^CCGGA
AcyI 386 GACGTC G[GA]^CG[CT]C
AfaI 30 GTAC GT^AC
AspHI 355 GAGCTC G[AT]GC[AT]^C
AvaII 291 GGACC G^G[AT]CC
BalI 647 TGGCCA TGG^CCA
BanI 222 GGTGCC G^G[CT][GA]CC
BbsI 567 GAAGAC GAAGAC(2/6)
BpuAI 567 GAAGAC GAAGAC(2/6)
BsaHI 386 GACGTC G[GA]^CG[CT]C
BseAI 419 TCCGGA T^CCGGA
BsiHKAI 355 GAGCTC G[AT]GC[AT]^C
BsmBI 384 GAGACG CGTCTC(1/5)
BspEI 419 TCCGGA T^CCGGA
BspMI 737 ACCTGC ACCTGC(4/8)
BstUI 823 CGCG CG^CG
BstYI 233 AGATCC [GA]^GATC[CT]
DraII 290 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 822 CCGCGG C^C[GA][CT]GG
EaeI 647 TGGCCA [CT]^GGCC[GA]
EcoO109I 290 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 689 CCAAGG C^C[AT][AT]GG
EcoT22I 544 ATGCAT ATGCA^T
Hin1I 386 GACGTC G[GA]^CG[CT]C
HphI 839 TCACC GGTGA(8/7)
KspI 822 CCGCGG CCGC^GG
MaeII 387 ACGT A^CGT
MflI 233 AGATCC [GA]^GATC[CT]
MluNI 647 TGGCCA TGG^CCA
MroI 419 TCCGGA T^CCGGA
MscI 647 TGGCCA TGG^CCA
MslI 658 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 823 CGCG CG^CG
NlaIV 222 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 544 ATGCAT ATGCA^T
PleI 184 GAGTC GAGTC(4/5)
PpuMI 290 AGGACCT [GA]G^G[AT]CC[CT]
PstI 739 CTGCAG CTGCA^G
RsaI 30 GTAC GT^AC
SacI 355 GAGCTC GAGCT^C
SacII 822 CCGCGG CCGC^GG
SapI 64 GAAGAGC GCTCTTC(1/4)
SbfI 738 CCTGCAGG CCTGCA^GG
SfcI 739 CTGCAG C^T[GA][CT]AG
Sse8387I 738 CCTGCAGG CCTGCA^GG
StyI 689 CCAAGG C^C[AT][AT]GG
TfiI 621 GATTC G^A[AT]TC
Tsp45I 838 GTCAC ^GT[GC]AC
XhoII 233 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwNI 101 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
307 CAGGCGCTG
BanII 355 GAGCTC G[GA]GC[CT]^C
467 GGGCTC
BbvCI 502 GCTGAGG CCTCAGC(-5/-2)
675 GCTGAGG
BcnI 515 CCCGG CC^[GC]GG
842 CCGGG
BmyI 355 GAGCTC G[AGT]GC[ACT]^C
467 GGGCTC
BsaI 109 GAGACC GGTCTC(1/5)
118 GAGACC
BsaWI 272 ACCGGA [AT]^CCGG[AT]
419 TCCGGA
Bsp1286I 355 GAGCTC G[AGT]GC[ACT]^C
467 GGGCTC
HaeII 309 GGCGCT [GA]GCGC^[CT]
845 GGCGCT
HinfI 184 GAGTC G^A[ACGT]TC
621 GATTC
MaeIII 557 GTTAC ^GT[ACGT]AC
838 GTCAC
NciI 515 CCCGG CC^[GC]GG
842 CCGGG
PvuII 56 CAGCTG CAG^CTG
673 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI BsiYI BslI BsmAI BsrBI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I