Restriction map for
pFN21AB5728
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AviII BbrPI BbvCI
BclI BfrI BglII BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NcoI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SnaBI SphI Sse8387I
SspBI SwaI Van91I XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 1623 GTCTAC GT^[AC][GT]AC
AccII 273 CGCG CG^CG
AcyI 210 GGCGCC G[GA]^CG[CT]C
AlwNI 1592 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 135 AGCGCT AGC^GCT
ApaI 186 GGGCCC GGGCC^C
Asp700I 571 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1351 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 315 CCTAGG C^CTAGG
BamHI 1572 GGATCC G^GATCC
BanI 210 GGCGCC G^G[CT][GA]CC
BbeI 210 GGCGCC GGCGC^C
BbsI 1093 GAAGAC GAAGAC(2/6)
BcgI 392 GCAACGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 549 GTATCC GTATCC(6/5)
BglI 179 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 315 CCTAGG C^CTAGG
BpuAI 1093 GAAGAC GAAGAC(2/6)
BsaHI 210 GGCGCC G[GA]^CG[CT]C
BsmI 1180 GAATGC GAATGC(1/-1)
BspMI 1402 ACCTGC ACCTGC(4/8)
BstBI 1410 TTCGAA TT^CGAA
BstUI 273 CGCG CG^CG
DraIII 625 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 135 AGCGCT AGC^GCT
EcoRI 796 GAATTC G^AATTC
Hin1I 210 GGCGCC G[GA]^CG[CT]C
HincII 1555 GTTGAC GT[CT]^[GA]AC
HindII 1555 GTTGAC GT[CT]^[GA]AC
KasI 210 GGCGCC G^GCGCC
MvnI 273 CGCG CG^CG
NarI 210 GGCGCC GG^CGCC
NdeI 1235 CATATG CA^TATG
NheI 1459 GCTAGC G^CTAGC
NspI 768 ACATGC [GA]CATG^[CT]
NspV 1410 TTCGAA TT^CGAA
PflFI 1351 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2134 GAGTC GAGTC(4/5)
PpuMI 49 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1508 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI 781 CTACAG C^T[GA][CT]AG
SfiI 178 GGCCTCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 210 GGCGCC GGC^GCC
SfuI 1410 TTCGAA TT^CGAA
SgrAI 288 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 46 CCCGGG CCC^GGG
SmlI 802 CTCAAG C^T[CT][GA]AG
SpeI 732 ACTAGT A^CTAGT
SspI 535 AATATT AAT^ATT
StuI 1938 AGGCCT AGG^CCT
Tth111I 1351 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1632 TCTAGA T^CTAGA
XcmI 1700 CCAGATACCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 46 CCCGGG C^CCGGG
XmaI 46 CCCGGG C^CCGGG
XmnI 571 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 50 GGTCC G^G[AT]CC
621 GGACC
BsgI 736 GTGCAG GTGCAG(16/14)
1724 GTGCAG
BsiYI 283 CCCAGCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCTTGATTTGG
BslI 283 CCCAGCGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCTTGATTTGG
BsrFI 166 GCCGGC [GA]^CCGG[CT]
289 GCCGGC
BstYI 842 AGATCC [GA]^GATC[CT]
1572 GGATCC
Cfr10I 166 GCCGGC [GA]^CCGG[CT]
289 GCCGGC
DraII 49 GGGTCCT [GA]G^G[ACGT]CC[CT]
185 GGGGCCC
DsaI 116 CCGTGG C^C[GA][CT]GG
248 CCACGG
EcoO109I 49 GGGTCCT [GA]G^G[ACGT]CC[CT]
185 GGGGCCC
EcoT14I 315 CCTAGG C^C[AT][AT]GG
1039 CCTTGG
MaeII 1054 ACGT A^CGT
1899 ACGT
MflI 842 AGATCC [GA]^GATC[CT]
1572 GGATCC
MslI 1741 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1915 CATATCAATG
NaeI 166 GCCGGC GCC^GGC
289 GCCGGC
NgoMIV 166 GCCGGC G^CCGGC
289 GCCGGC
PvuII 267 CAGCTG CAG^CTG
1419 CAGCTG
StyI 315 CCTAGG C^C[AT][AT]GG
1039 CCTTGG
Tsp45I 70 GTGAC ^GT[GC]AC
1298 GTGAC
XhoII 842 AGATCC [GA]^GATC[CT]
1572 GGATCC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI BalI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I