Restriction map for
pFN21AE9414
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlpI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacI
SacII SalI Sau96I SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 1005 GTGCAC G^TGCAC
ApaLI 1005 GTGCAC G^TGCAC
AvaI 869 CCCGGG C^[CT]CG[GA]G
AvrII 1054 CCTAGG C^CTAGG
BalI 93 TGGCCA TGG^CCA
BamHI 29 GGATCC G^GATCC
BanII 722 GAGCCC G[GA]GC[CT]^C
BbsI 986 GAAGAC GAAGAC(2/6)
BclI 158 TGATCA T^GATCA
BlnI 1054 CCTAGG C^CTAGG
BpuAI 986 GAAGAC GAAGAC(2/6)
BsoBI 869 CCCGGG C^[CT]CG[GA]G
BspHI 738 TCATGA T^CATGA
BsrDI 162 CATTGC GCAATG(2/0)
BssSI 325 CACGAG CACGAG(-5/-1)
BstAPI 388 GCATGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 342 CCAGGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 93 TGGCCA [CT]^GGCC[GA]
EarI 719 GAAGAG CTCTTC(1/4)
FbaI 158 TGATCA T^GATCA
HgaI 69 GCGTC GACGC(5/10)
Ksp632I 719 GAAGAG CTCTTC(1/4)
MaeII 583 ACGT A^CGT
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MseI 901 TTAA T^TAA
NdeI 251 CATATG CA^TATG
NspI 388 GCATGT [GA]CATG^[CT]
PflMI 581 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 328 GAGTC GAGTC(4/5)
RcaI 738 TCATGA T^CATGA
SapI 719 GAAGAGC GCTCTTC(1/4)
SexAI 546 ACCTGGT A^CC[AT]GGT
SfcI 99 CTACAG C^T[GA][CT]AG
SmaI 869 CCCGGG CCC^GGG
SmlI 471 CTTGAG C^T[CT][GA]AG
StuI 978 AGGCCT AGG^CCT
TaqI 125 TCGA T^CGA
Tru9I 901 TTAA T^TAA
TthHB8I 125 TCGA T^CGA
Van91I 581 CCACGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 858 TCTAGA T^CTAGA
XmaCI 869 CCCGGG C^CCGGG
XmaI 869 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 476 GAATTT [GA]^AATT[CT]
683 AAATTT
ApoI 476 GAATTT [GA]^AATT[CT]
683 AAATTT
AspHI 200 GTGCTC G[AT]GC[AT]^C
1005 GTGCAC
BbvI 278 GCTGC GCAGC(8/12)
643 GCTGC
BcnI 869 CCCGG CC^[GC]GG
870 CCGGG
BsaBI 412 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
492 GATCTGGATC
BsiHKAI 200 GTGCTC G[AT]GC[AT]^C
1005 GTGCAC
BstEII 456 GGTCACC G^GT[ACGT]ACC
550 GGTTACC
BstF5I 1010 CATCC GGATG(2/0)
111 GGATG
BstPI 456 GGTCACC G^GT[ACGT]ACC
550 GGTTACC
BstYI 29 GGATCC [GA]^GATC[CT]
497 GGATCT
Cac8I 397 GCTTGC GC[ACGT]^[ACGT]GC
912 GCTTGC
DdeI 363 CTCAG C^T[ACGT]AG
995 CTGAG
DsaI 555 CCATGG C^C[GA][CT]GG
791 CCATGG
EcoO65I 456 GGTCACC G^GT[ACGT]ACC
550 GGTTACC
Fnu4HI 278 GCTGC GC^[ACGT]GC
643 GCTGC
FokI 1010 CATCC GGATG(9/13)
111 GGATG
HaeIII 94 GGCC GG^CC
979 GGCC
HapII 870 CCGG C^CGG
972 CCGG
HpaII 870 CCGG C^CGG
972 CCGG
ItaI 278 GCTGC GC^[ACGT]GC
643 GCTGC
MamI 412 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
492 GATCTGGATC
MflI 29 GGATCC [GA]^GATC[CT]
497 GGATCT
MslI 97 CACTACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
402 CATCAGTGTG
MspI 870 CCGG C^CGG
972 CCGG
MwoI 388 GCATGTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
637 GCTCCTGCTGC
NciI 869 CCCGG CC^[GC]GG
870 CCGGG
NcoI 555 CCATGG C^CATGG
791 CCATGG
SfaNI 119 GCATC GCATC(5/9)
401 GCATC
TseI 278 GCTGC G^C[AT]GC
643 GCTGC
XhoII 29 GGATCC [GA]^GATC[CT]
497 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AluI AlwI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI DpnII EcoRII EcoT14I
HinfI HphI MaeI MaeIII MboI
MboII MnlI MvaI NdeII NlaIII
NlaIV Sau3AI ScrFI StyI TfiI
Tsp45I Tsp509I TspRI