Restriction map for
pFN21AE9617
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvaI AviII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FauI FbaI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MboI MflI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp45I XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AfaI 727 GTAC GT^AC
Alw44I 14 GTGCAC G^TGCAC
ApaLI 14 GTGCAC G^TGCAC
Asp700I 403 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 14 GTGCAC G[AT]GC[AT]^C
AspI 796 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 372 CCTAGG C^CTAGG
BanI 67 GGTGCC G^G[CT][GA]CC
BbvI 664 GCTGC GCAGC(8/12)
BcnI 399 CCCGG CC^[GC]GG
BlnI 372 CCTAGG C^CTAGG
BmyI 14 GTGCAC G[AGT]GC[ACT]^C
BpmI 511 CTGGAG CTGGAG(16/14)
BsaBI 89 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 74 CTCCTC GAGGAG(10/8)
BsiHKAI 14 GTGCAC G[AT]GC[AT]^C
BsmI 193 GCATTC GAATGC(1/-1)
Bsp1286I 14 GTGCAC G[AGT]GC[ACT]^C
BstAPI 634 GCAATCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 822 GGTTACC G^GT[ACGT]ACC
BstPI 822 GGTTACC G^GT[ACGT]ACC
Cac8I 679 GCATGC GC[ACGT]^[ACGT]GC
DdeI 446 CTGAG C^T[ACGT]AG
DraII 395 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 395 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 822 GGTTACC G^GT[ACGT]ACC
EcoT14I 372 CCTAGG C^C[AT][AT]GG
EcoT22I 641 ATGCAT ATGCA^T
Fnu4HI 664 GCTGC GC^[ACGT]GC
HapII 400 CCGG C^CGG
HpaII 400 CCGG C^CGG
HphI 533 TCACC GGTGA(8/7)
ItaI 664 GCTGC GC^[ACGT]GC
MaeIII 823 GTTAC ^GT[ACGT]AC
MamI 89 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 436 CAATTG C^AATTG
MspI 400 CCGG C^CGG
MunI 436 CAATTG C^AATTG
NciI 399 CCCGG CC^[GC]GG
NsiI 641 ATGCAT ATGCA^T
NspI 679 GCATGC [GA]CATG^[CT]
PflFI 796 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 693 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 395 GGGACCC [GA]G^G[AT]CC[CT]
PstI 79 CTGCAG CTGCA^G
RsaI 727 GTAC GT^AC
SmlI 42 CTCAAG C^T[CT][GA]AG
SphI 679 GCATGC GCATG^C
StyI 372 CCTAGG C^C[AT][AT]GG
TaqI 135 TCGA T^CGA
TseI 664 GCTGC G^C[AT]GC
Tth111I 796 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 135 TCGA T^CGA
Van91I 693 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 274 TCTAGA T^CTAGA
XcmI 804 CCACAAAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 403 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AluI 786 AGCT AG^CT
933 AGCT
AvaII 396 GGACC G^G[AT]CC
690 GGACC
BbsI 337 GAAGAC GAAGAC(2/6)
506 GTCTTC
BpuAI 337 GAAGAC GAAGAC(2/6)
506 GTCTTC
BsmAI 500 GAGAC GTCTC(1/5)
514 GAGAC
BsmFI 395 GGGAC GGGAC(10/14)
794 GGGAC
Cfr13I 396 GGACC G^G[ACGT]CC
690 GGACC
MseI 229 TTAA T^TAA
647 TTAA
MslI 348 CATCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
943 CATACTGATG
Sau96I 396 GGACC G^G[ACGT]CC
690 GGACC
SfaNI 778 GATGC GCATC(5/9)
37 GCATC
Tru9I 229 TTAA T^TAA
647 TTAA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI ApoI BfaI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
EcoRII FokI HincII HindII HinfI
MaeI MboII MnlI MvaI MwoI
NlaIII NlaIV PleI ScrFI SfcI
TfiI Tsp509I TspRI