Restriction map for
pFN21AE6041
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglII BlnI BmyI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII KasI KpnI KspI MaeII
MamI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 216 GTATAC GT^[AC][GT]AC
AciI 780 GCGG CCGC(-3/-1)
AcsI 31 GAATTT [GA]^AATT[CT]
AlwNI 69 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTT [GA]^AATT[CT]
AseI 604 ATTAAT AT^TAAT
AsnI 604 ATTAAT AT^TAAT
BalI 371 TGGCCA TGG^CCA
BbsI 872 GTCTTC GAAGAC(2/6)
BfaI 935 CTAG C^TAG
BglI 424 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 552 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
BpuAI 872 GTCTTC GAAGAC(2/6)
BsgI 163 CTGCAC GTGCAG(16/14)
BsmFI 464 GTCCC GGGAC(10/14)
BsmI 331 GAATGC GAATGC(1/-1)
BspHI 641 TCATGA T^CATGA
Bst1107I 216 GTATAC GTA^TAC
BstZ17I 216 GTATAC GTA^TAC
CelII 552 GCTGAGC GC^T[ACGT]AGC
DraIII 416 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EarI 789 GAAGAG CTCTTC(1/4)
HgaI 325 GCGTC GACGC(5/10)
HphI 110 TCACC GGTGA(8/7)
Ksp632I 789 GAAGAG CTCTTC(1/4)
MaeI 935 CTAG C^TAG
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
NcoI 350 CCATGG C^CATGG
NlaIV 383 GGCCCC GG[ACGT]^[ACGT]CC
NspI 125 ACATGC [GA]CATG^[CT]
PflMI 312 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 604 ATTAAT AT^TAAT
RcaI 641 TCATGA T^CATGA
SapI 789 GAAGAGC GCTCTTC(1/4)
SspI 250 AATATT AAT^ATT
StuI 707 AGGCCT AGG^CCT
Tsp45I 536 GTGAC ^GT[GC]AC
Van91I 312 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 177 GTAC GT^AC
838 GTAC
AlwI 3 GGATC GGATC(4/5)
659 GGATC
AvaII 741 GGTCC G^G[AT]CC
908 GGACC
BsiYI 312 CCACTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
374 CCATTTGTCGG
BslI 312 CCACTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
374 CCATTTGTCGG
BsrI 150 ACTGG ACTGG(1/-1)
53 CCAGT
BssKI 24 CCTGG ^CC[ACGT]GG
369 CCTGG
BstNI 24 CCTGG CC^[AT]GG
369 CCTGG
Cac8I 74 GCTGGC GC[ACGT]^[ACGT]GC
562 GCCTGC
DpnII 4 GATC ^GATC
660 GATC
EcoRII 24 CCTGG ^CC[AT]GG
369 CCTGG
MboI 4 GATC ^GATC
660 GATC
MseI 605 TTAA T^TAA
893 TTAA
MvaI 24 CCTGG CC^[AT]GG
369 CCTGG
NdeII 4 GATC ^GATC
660 GATC
RsaI 177 GTAC GT^AC
838 GTAC
Sau3AI 4 GATC ^GATC
660 GATC
ScrFI 24 CCTGG CC^[ACGT]GG
369 CCTGG
SfcI 824 CTATAG C^T[GA][CT]AG
867 CTACAG
Tru9I 605 TTAA T^TAA
893 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BbvI BsaJI BstF5I Cfr13I
DdeI EcoT14I Fnu4HI FokI HaeIII
ItaI MaeIII MboII MnlI MslI
MwoI NlaIII Sau96I SfaNI StyI
TseI Tsp509I TspRI