Restriction map for
pFN21AE5490
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AvrII BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BmyI BpuAI BsaAI BsaBI
BsaHI BsaI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MamI MluI MseI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StyI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 737 TCCGGA T^CCGGA
AviII 141 TGCGCA TGC^GCA
BalI 437 TGGCCA TGG^CCA
BamHI 523 GGATCC G^GATCC
BciVI 506 GTATCC GTATCC(6/5)
BfaI 664 CTAG C^TAG
BglII 293 AGATCT A^GATCT
BlpI 636 GCTGAGC GC^T[ACGT]AGC
BpmI 289 CTGGAG CTGGAG(16/14)
Bpu1102I 636 GCTGAGC GC^T[ACGT]AGC
BsaJI 362 CCGTGG C^C[ACGT][ACGT]GG
BsaWI 737 TCCGGA [AT]^CCGG[AT]
BseAI 737 TCCGGA T^CCGGA
BsmAI 44 GTCTC GTCTC(1/5)
BsmFI 890 GGGAC GGGAC(10/14)
BsmI 761 GAATGC GAATGC(1/-1)
BspEI 737 TCCGGA T^CCGGA
BspHI 488 TCATGA T^CATGA
BstXI 396 CCACTCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 636 GCTGAGC GC^T[ACGT]AGC
CfoI 142 GCGC GCG^C
Cfr13I 243 GGCCC G^G[ACGT]CC
DsaI 362 CCGTGG C^C[GA][CT]GG
FauI 135 CCCGC CCCGC(4/6)
FspI 141 TGCGCA TGC^GCA
HapII 738 CCGG C^CGG
HhaI 142 GCGC GCG^C
HinP1I 142 GCGC G^CGC
HincII 81 GTTGAC GT[CT]^[GA]AC
HindII 81 GTTGAC GT[CT]^[GA]AC
HpaII 738 CCGG C^CGG
MaeI 664 CTAG C^TAG
MfeI 899 CAATTG C^AATTG
MluNI 437 TGGCCA TGG^CCA
MroI 737 TCCGGA T^CCGGA
MscI 437 TGGCCA TGG^CCA
MspI 738 CCGG C^CGG
MunI 899 CAATTG C^AATTG
NspI 138 GCATGC [GA]CATG^[CT]
PleI 42 GAGTC GAGTC(4/5)
PstI 379 CTGCAG CTGCA^G
RcaI 488 TCATGA T^CATGA
SapI 351 GAAGAGC GCTCTTC(1/4)
Sau96I 243 GGCCC G^G[ACGT]CC
SphI 138 GCATGC GCATG^C
SspI 8 AATATT AAT^ATT
StuI 788 AGGCCT AGG^CCT
XcmI 510 CCAGCTGACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AciI 136 CCGC CCGC(-3/-1)
694 GCGG
AfaI 387 GTAC GT^AC
450 GTAC
BsgI 247 CTGCAC GTGCAG(16/14)
648 CTGCAC
BsrDI 720 CATTGC GCAATG(2/0)
433 GCAATG
BsrI 202 ACTGG ACTGG(1/-1)
89 CCAGT
EaeI 86 CGGCCA [CT]^GGCC[GA]
437 TGGCCA
HphI 108 GGTGA GGTGA(8/7)
810 TCACC
NlaIV 523 GGATCC GG[ACGT]^[ACGT]CC
592 GGCTCC
PvuII 511 CAGCTG CAG^CTG
770 CAGCTG
RsaI 387 GTAC GT^AC
450 GTAC
SfaNI 324 GATGC GCATC(5/9)
679 GATGC
SfcI 379 CTGCAG C^T[GA][CT]AG
948 CTATAG
Tsp45I 109 GTGAC ^GT[GC]AC
907 GTCAC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AlwI BbvI BsiYI BslI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MvaI MwoI NdeII
NlaIII Sau3AI ScrFI TfiI TseI
Tsp509I TspRI XhoII