Restriction map for
pFN21AE6023
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp700I Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BfaI BfrI BglI BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspLU11I BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MspA1I MunI MvnI
MwoI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AciI 8 GCGG CCGC(-3/-1)
AcsI 31 GAATTT [GA]^AATT[CT]
AflIII 572 ACACGT A^C[GA][CT]GT
AluI 56 AGCT AG^CT
AlwI 335 GGATC GGATC(4/5)
AlwNI 23 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 508 GGGCCC GGGCC^C
ApoI 31 GAATTT [GA]^AATT[CT]
AseI 129 ATTAAT AT^TAAT
AsnI 129 ATTAAT AT^TAAT
AspHI 422 GTGCTC G[AT]GC[AT]^C
AspI 538 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 472 CCCGAG C^[CT]CG[GA]G
AvaII 543 GGTCC G^G[AT]CC
BalI 353 TGGCCA TGG^CCA
BanII 508 GGGCCC G[GA]GC[CT]^C
BbvCI 52 CCTCAGC CCTCAGC(-5/-2)
BbvI 287 GCTGC GCAGC(8/12)
BclI 401 TGATCA T^GATCA
BcnI 413 CCCGG CC^[GC]GG
BlpI 552 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 552 GCTAAGC GC^T[ACGT]AGC
BsgI 719 GTGCAG GTGCAG(16/14)
BsiHKAI 422 GTGCTC G[AT]GC[AT]^C
BsmAI 71 GTCTC GTCTC(1/5)
BsmFI 544 GTCCC GGGAC(10/14)
BsoBI 472 CCCGAG C^[CT]CG[GA]G
Bsp1407I 565 TGTACA T^GTACA
BspHI 404 TCATGA T^CATGA
BspMI 436 GCAGGT ACCTGC(4/8)
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BsrGI 565 TGTACA T^GTACA
Cac8I 721 GCAGGC GC[ACGT]^[ACGT]GC
CelII 552 GCTAAGC GC^T[ACGT]AGC
DsaI 350 CCATGG C^C[GA][CT]GG
EaeI 353 TGGCCA [CT]^GGCC[GA]
EcoT14I 350 CCATGG C^C[AT][AT]GG
FbaI 401 TGATCA T^GATCA
Fnu4HI 287 GCTGC GC^[ACGT]GC
HapII 414 CCGG C^CGG
HpaII 414 CCGG C^CGG
ItaI 287 GCTGC GC^[ACGT]GC
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MspI 414 CCGG C^CGG
NciI 413 CCCGG CC^[GC]GG
NcoI 350 CCATGG C^CATGG
PflFI 538 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 129 ATTAAT AT^TAAT
PstI 434 CTGCAG CTGCA^G
RcaI 404 TCATGA T^CATGA
SfcI 434 CTGCAG C^T[GA][CT]AG
SspBI 565 TGTACA T^GTACA
StyI 350 CCATGG C^C[AT][AT]GG
TseI 287 GCTGC G^C[AT]GC
Tth111I 538 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 726 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbsI 736 GTCTTC GAAGAC(2/6)
914 GTCTTC
BglII 209 AGATCT A^GATCT
527 AGATCT
BmyI 422 GTGCTC G[AGT]GC[ACT]^C
508 GGGCCC
BpmI 205 CTGGAG CTGGAG(16/14)
938 CTGGAG
BpuAI 736 GTCTTC GAAGAC(2/6)
914 GTCTTC
BsaJI 350 CCATGG C^C[ACGT][ACGT]GG
412 CCCCGG
BsiYI 499 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
726 CCACCTCATGG
BslI 499 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
726 CCACCTCATGG
Bsp1286I 422 GTGCTC G[AGT]GC[ACT]^C
508 GGGCCC
BsrDI 636 CATTGC GCAATG(2/0)
816 CATTGC
BstF5I 63 GGATG GGATG(2/0)
594 GGATG
BstNI 558 CCTGG CC^[AT]GG
937 CCTGG
EcoRII 558 CCTGG ^CC[AT]GG
937 CCTGG
FokI 63 GGATG GGATG(9/13)
594 GGATG
MaeII 368 ACGT A^CGT
574 ACGT
MseI 130 TTAA T^TAA
899 TTAA
MslI 273 CATCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
573 CACGTCCATG
MvaI 558 CCTGG CC^[AT]GG
937 CCTGG
SfaNI 240 GATGC GCATC(5/9)
290 GCATC
Tru9I 130 TTAA T^TAA
899 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI BsrI BssKI BstYI Cfr13I
DdeI DpnII EarI HaeIII HinfI
Ksp632I MaeIII MboI MboII MflI
MnlI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TfiI Tsp45I
Tsp509I TspRI XhoII