Restriction map for
pFN21AE6086
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrGI BssHII BstAPI
BstEII BstPI BstUI CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflIII 721 ACGTGT A^C[GA][CT]GT
AlwI 482 GGATC GGATC(4/5)
AocI 876 CCTGAGG CC^T[ACGT]AGG
AspHI 191 GTGCTC G[AT]GC[AT]^C
BalI 368 TGGCCA TGG^CCA
BanII 6 GAGCCC G[GA]GC[CT]^C
BbrPI 720 CACGTG CAC^GTG
BfaI 248 CTAG C^TAG
BsaBI 636 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 264 GAGGAG GAGGAG(10/8)
BsgI 561 CTGCAC GTGCAG(16/14)
BsiHKAI 191 GTGCTC G[AT]GC[AT]^C
BsmFI 952 GGGAC GGGAC(10/14)
BsrDI 822 CATTGC GCAATG(2/0)
BsrFI 316 ACCGGC [GA]^CCGG[CT]
BssSI 572 CACGAG CACGAG(-5/-1)
BstBI 507 TTCGAA TT^CGAA
BstF5I 600 GGATG GGATG(2/0)
BstXI 821 CCATTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 482 GGATCT [GA]^GATC[CT]
Bsu36I 876 CCTGAGG CC^T[ACGT]AGG
Cfr10I 316 ACCGGC [GA]^CCGG[CT]
DpnII 483 GATC ^GATC
DraII 952 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 368 TGGCCA [CT]^GGCC[GA]
Eco81I 876 CCTGAGG CC^T[ACGT]AGG
EcoO109I 952 GGGACCT [GA]G^G[ACGT]CC[CT]
FokI 600 GGATG GGATG(9/13)
HapII 317 CCGG C^CGG
HgaI 552 GCGTC GACGC(5/10)
HpaII 317 CCGG C^CGG
MaeI 248 CTAG C^TAG
MamI 636 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 483 GATC ^GATC
MflI 482 GGATCT [GA]^GATC[CT]
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MspI 317 CCGG C^CGG
NdeI 372 CATATG CA^TATG
NdeII 483 GATC ^GATC
NlaIV 952 GGGACC GG[ACGT]^[ACGT]CC
NspV 507 TTCGAA TT^CGAA
PleI 768 GACTC GAGTC(4/5)
PmaCI 720 CACGTG CAC^GTG
PmlI 720 CACGTG CAC^GTG
PpuMI 952 GGGACCT [GA]G^G[AT]CC[CT]
PstI 292 CTGCAG CTGCA^G
Sau3AI 483 GATC ^GATC
SexAI 125 ACCTGGT A^CC[AT]GGT
SfuI 507 TTCGAA TT^CGAA
SmlI 957 CTCAAG C^T[CT][GA]AG
SnaBI 680 TACGTA TAC^GTA
Tsp509I 675 AATT ^AATT
XhoII 482 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 177 GTATAC GT^[AC][GT]AC
216 GTATAC
AfaI 419 GTAC GT^AC
777 GTAC
AvaII 144 GGTCC G^G[AT]CC
953 GGACC
BbvI 377 GCAGC GCAGC(8/12)
549 GCTGC
BmyI 6 GAGCCC G[AGT]GC[ACT]^C
191 GTGCTC
BpmI 205 CTGGAG CTGGAG(16/14)
695 CTGGAG
BsaAI 680 TACGTA [CT]AC^GT[GA]
720 CACGTG
BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG
949 CCAGGG
BsiYI 360 CCACTACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
496 CCATTTTGTGG
BslI 360 CCACTACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
496 CCATTTTGTGG
Bsp1286I 6 GAGCCC G[AGT]GC[ACT]^C
191 GTGCTC
BspHI 401 TCATGA T^CATGA
584 TCATGA
BsrI 147 CCAGT ACTGG(1/-1)
544 CCAGT
Bst1107I 177 GTATAC GTA^TAC
216 GTATAC
BstZ17I 177 GTATAC GTA^TAC
216 GTATAC
Cac8I 251 GCCTGC GC[ACGT]^[ACGT]GC
559 GCCTGC
Cfr13I 144 GGTCC G^G[ACGT]CC
953 GGACC
HphI 159 TCACC GGTGA(8/7)
513 TCACC
MaeII 681 ACGT A^CGT
721 ACGT
MaeIII 533 GTGAC ^GT[ACGT]AC
766 GTGAC
MseI 65 TTAA T^TAA
433 TTAA
MspA1I 629 CAGCTG C[AC]G^C[GT]G
692 CAGCTG
PflMI 360 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
496 CCATTTTGTGG
PvuII 629 CAGCTG CAG^CTG
692 CAGCTG
RcaI 401 TCATGA T^CATGA
584 TCATGA
RsaI 419 GTAC GT^AC
777 GTAC
SapI 93 GCTCTTC GCTCTTC(1/4)
750 GCTCTTC
Sau96I 144 GGTCC G^G[ACGT]CC
953 GGACC
SfcI 292 CTGCAG C^T[GA][CT]AG
870 CTATAG
TaqI 15 TCGA T^CGA
508 TCGA
TfiI 510 GAATC G^A[AT]TC
588 GATTC
Tru9I 65 TTAA T^TAA
433 TTAA
TseI 377 GCAGC G^C[AT]GC
549 GCTGC
Tsp45I 533 GTGAC ^GT[GC]AC
766 GTGAC
TthHB8I 15 TCGA T^CGA
508 TCGA
Van91I 360 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
496 CCATTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BsmAI BssKI BstNI
DdeI EarI EcoRII Fnu4HI HaeIII
HinfI ItaI Ksp632I MboII MnlI
MslI MvaI NlaIII ScrFI SfaNI
TspRI