Restriction map for
pFN21AE1601
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeII MamI MboII MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TseI Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 107 GGCGTC G[GA]^CG[CT]C
AluI 114 AGCT AG^CT
AlwI 137 GGATC GGATC(4/5)
Asp700I 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 223 GAGCAC G[AT]GC[AT]^C
AspI 205 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 112 CTAG C^TAG
BlpI 46 GCTAAGC GC^T[ACGT]AGC
BmyI 223 GAGCAC G[AGT]GC[ACT]^C
BpmI 187 CTGGAG CTGGAG(16/14)
Bpu1102I 46 GCTAAGC GC^T[ACGT]AGC
BsaHI 107 GGCGTC G[GA]^CG[CT]C
BsaI 160 GAGACC GGTCTC(1/5)
BseRI 157 GAGGAG GAGGAG(10/8)
BsiHKAI 223 GAGCAC G[AT]GC[AT]^C
BsiYI 54 CCACCGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 54 CCACCGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 203 GGGAC GGGAC(10/14)
Bsp1286I 223 GAGCAC G[AGT]GC[ACT]^C
BstF5I 255 GGATG GGATG(2/0)
BstXI 251 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 137 GGATCT [GA]^GATC[CT]
CelII 46 GCTAAGC GC^T[ACGT]AGC
DpnII 138 GATC ^GATC
EcoT14I 251 CCATGG C^C[AT][AT]GG
FauI 164 CCCGC CCCGC(4/6)
Fnu4HI 125 GCGGC GC^[ACGT]GC
FokI 255 GGATG GGATG(9/13)
HaeIII 145 GGCC GG^CC
HapII 5 CCGG C^CGG
Hin1I 107 GGCGTC G[GA]^CG[CT]C
HinfI 198 GATTC G^A[ACGT]TC
HpaII 5 CCGG C^CGG
ItaI 125 GCGGC GC^[ACGT]GC
KspI 165 CCGCGG CCGC^GG
MaeI 112 CTAG C^TAG
MboI 138 GATC ^GATC
MflI 137 GGATCT [GA]^GATC[CT]
MseI 131 TTAA T^TAA
MspA1I 165 CCGCGG C[AC]G^C[GT]G
MspI 5 CCGG C^CGG
NcoI 251 CCATGG C^CATGG
NdeII 138 GATC ^GATC
NlaIII 252 CATG CATG^
PflFI 205 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 165 CCGCGG CCGC^GG
Sau3AI 138 GATC ^GATC
SfaNI 86 GCATC GCATC(5/9)
StyI 251 CCATGG C^C[AT][AT]GG
TfiI 198 GATTC G^A[AT]TC
Tru9I 131 TTAA T^TAA
Tth111I 205 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 137 GGATCT [GA]^GATC[CT]
XmnI 177 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 166 CGCG CG^CG
267 CGCG
AfaI 264 GTAC GT^AC
294 GTAC
BsmAI 71 GAGAC GTCTC(1/5)
160 GAGAC
BssKI 186 CCTGG ^CC[ACGT]GG
279 CCAGG
BstNI 186 CCTGG CC^[AT]GG
279 CCAGG
BstUI 166 CGCG CG^CG
267 CGCG
Cac8I 96 GCCTGC GC[ACGT]^[ACGT]GC
225 GCACGC
DdeI 30 CTTAG C^T[ACGT]AG
47 CTAAG
DsaI 165 CCGCGG C^C[GA][CT]GG
251 CCATGG
EcoRII 186 CCTGG ^CC[AT]GG
279 CCAGG
HgaI 205 GACGC GACGC(5/10)
108 GCGTC
HphI 89 TCACC GGTGA(8/7)
212 TCACC
MaeIII 211 GTCAC ^GT[ACGT]AC
244 GTCAC
MslI 246 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
252 CATGGATGTG
MvaI 186 CCTGG CC^[AT]GG
279 CCAGG
MvnI 166 CGCG CG^CG
267 CGCG
RsaI 264 GTAC GT^AC
294 GTAC
ScrFI 186 CCTGG CC^[ACGT]GG
279 CCAGG
SmlI 66 CTTGAG C^T[CT][GA]AG
271 CTCAAG
Tsp45I 211 GTCAC ^GT[GC]AC
244 GTCAC
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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AciI BsaJI CfoI HaeII HhaI
HinP1I MnlI MwoI