Restriction map for
pFN21AE6174
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MluNI MscI
MslI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TseI Tsp45I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 37 TCCGGA T^CCGGA
AciI 144 GCGG CCGC(-3/-1)
AocI 46 CCTGAGG CC^T[ACGT]AGG
AvaI 137 CCCGGG C^[CT]CG[GA]G
AvaII 234 GGACC G^G[AT]CC
BamHI 81 GGATCC G^GATCC
BciVI 169 GTATCC GTATCC(6/5)
BfaI 148 CTAG C^TAG
BpmI 7 CTGGAG CTGGAG(16/14)
BsaJI 137 CCCGGG C^C[ACGT][ACGT]GG
BsaWI 37 TCCGGA [AT]^CCGG[AT]
BseAI 37 TCCGGA T^CCGGA
BsoBI 137 CCCGGG C^[CT]CG[GA]G
BspEI 37 TCCGGA T^CCGGA
BsrBI 142 GAGCGG CCGCTC(-3/-3)
BsrI 6 ACTGG ACTGG(1/-1)
BstAPI 18 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 46 CCTGAGG CC^T[ACGT]AGG
DdeI 47 CTGAG C^T[ACGT]AG
Eco81I 46 CCTGAGG CC^T[ACGT]AGG
Fnu4HI 144 GCGGC GC^[ACGT]GC
HinfI 34 GAATC G^A[ACGT]TC
ItaI 144 GCGGC GC^[ACGT]GC
MaeI 148 CTAG C^TAG
MroI 37 TCCGGA T^CCGGA
MseI 260 TTAA T^TAA
NlaIII 226 CATG CATG^
NspI 225 GCATGC [GA]CATG^[CT]
PflMI 237 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfaNI 24 GATGC GCATC(5/9)
SmaI 137 CCCGGG CCC^GGG
SphI 225 GCATGC GCATG^C
TaqI 177 TCGA T^CGA
TfiI 34 GAATC G^A[AT]TC
Tru9I 260 TTAA T^TAA
Tsp509I 74 AATT ^AATT
TspRI 95 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 177 TCGA T^CGA
Van91I 237 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 137 CCCGGG C^CCGGG
XmaI 137 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AluI 152 AGCT AG^CT
209 AGCT
AspHI 151 GAGCTC G[AT]GC[AT]^C
208 GAGCTC
BanII 151 GAGCTC G[GA]GC[CT]^C
208 GAGCTC
BcnI 137 CCCGG CC^[GC]GG
138 CCGGG
BmyI 151 GAGCTC G[AGT]GC[ACT]^C
208 GAGCTC
BsiHKAI 151 GAGCTC G[AT]GC[AT]^C
208 GAGCTC
BsiYI 137 CCCGGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCATTGCGTGG
BslI 137 CCCGGGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCATTGCGTGG
BsmFI 3 GGGAC GGGAC(10/14)
233 GGGAC
Bsp1286I 151 GAGCTC G[AGT]GC[ACT]^C
208 GAGCTC
BsrDI 238 CATTGC GCAATG(2/0)
105 GCAATG
BssKI 137 CCCGG ^CC[ACGT]GG
138 CCGGG
BstYI 42 AGATCC [GA]^GATC[CT]
81 GGATCC
Cac8I 110 GCGAGC GC[ACGT]^[ACGT]GC
225 GCATGC
HaeIII 135 GGCC GG^CC
246 GGCC
HapII 38 CCGG C^CGG
138 CCGG
HpaII 38 CCGG C^CGG
138 CCGG
MflI 42 AGATCC [GA]^GATC[CT]
81 GGATCC
MspI 38 CCGG C^CGG
138 CCGG
NciI 137 CCCGG CC^[GC]GG
138 CCGGG
NlaIV 81 GGATCC GG[ACGT]^[ACGT]CC
233 GGGACC
SacI 151 GAGCTC GAGCT^C
208 GAGCTC
ScrFI 137 CCCGG CC^[ACGT]GG
138 CCGGG
XhoII 42 AGATCC [GA]^GATC[CT]
81 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AlwI BseRI Cfr13I DpnII EarI
Ksp632I MboI MboII MnlI MwoI
NdeII Sau3AI Sau96I