Restriction map for
pFN21AE9401
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrFI BssHII
Bst1107I BstBI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FseI FspI
HindIII HpaI KasI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 1290 GGTACC G^GTACC
AflIII 47 ACACGT A^C[GA][CT]GT
AlwI 381 GGATC GGATC(4/5)
AlwNI 181 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1237 GGGCCC GGGCC^C
Asp718I 1290 GGTACC G^GTACC
AspHI 486 GAGCAC G[AT]GC[AT]^C
AvaII 875 GGACC G^G[AT]CC
BanII 1237 GGGCCC G[GA]GC[CT]^C
BbrPI 48 CACGTG CAC^GTG
BbsI 1069 GAAGAC GAAGAC(2/6)
BclI 1304 TGATCA T^GATCA
BfaI 160 CTAG C^TAG
BpuAI 1069 GAAGAC GAAGAC(2/6)
BsaAI 48 CACGTG [CT]AC^GT[GA]
BsaBI 1200 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 486 GAGCAC G[AT]GC[AT]^C
BsmAI 690 GAGAC GTCTC(1/5)
Bsp1407I 677 TGTACA T^GTACA
BspHI 620 TCATGA T^CATGA
BsrBI 99 CCGCTC CCGCTC(-3/-3)
BsrDI 533 GCAATG GCAATG(2/0)
BsrGI 677 TGTACA T^GTACA
BssSI 247 CTCGTG CACGAG(-5/-1)
DdeI 583 CTCAG C^T[ACGT]AG
FbaI 1304 TGATCA T^GATCA
HgaI 353 GCGTC GACGC(5/10)
KpnI 1290 GGTACC GGTAC^C
KspI 438 CCGCGG CCGC^GG
MaeI 160 CTAG C^TAG
MamI 1200 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 548 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 710 GAGTC GAGTC(4/5)
PmaCI 48 CACGTG CAC^GTG
PmlI 48 CACGTG CAC^GTG
PshAI 1003 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 181 CAGCTG CAG^CTG
RcaI 620 TCATGA T^CATGA
SacII 438 CCGCGG CCGC^GG
SspBI 677 TGTACA T^GTACA
TfiI 1507 GATTC G^A[AT]TC
Tsp509I 1081 AATT ^AATT
Van91I 548 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 496 CCACTCTACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
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AatII 3 GACGTC GACGT^C
1096 GACGTC
AcyI 3 GACGTC G[GA]^CG[CT]C
1096 GACGTC
AfaI 678 GTAC GT^AC
1291 GTAC
Asp700I 1407 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1410 GAAGTTCTTC
BanI 460 GGCACC G^G[CT][GA]CC
1290 GGTACC
BmyI 486 GAGCAC G[AGT]GC[ACT]^C
1237 GGGCCC
BsaHI 3 GACGTC G[GA]^CG[CT]C
1096 GACGTC
Bsp1286I 486 GAGCAC G[AGT]GC[ACT]^C
1237 GGGCCC
BstAPI 180 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
666 GCATGCTCTGC
BstEII 192 GGTGACC G^GT[ACGT]ACC
442 GGTTACC
BstPI 192 GGTGACC G^GT[ACGT]ACC
442 GGTTACC
BstXI 74 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
362 CCATTGTCGTGG
DraII 1236 GGGGCCC [GA]G^G[ACGT]CC[CT]
1237 GGGCCCT
EarI 283 CTCTTC CTCTTC(1/4)
739 GAAGAG
EcoO109I 1236 GGGGCCC [GA]G^G[ACGT]CC[CT]
1237 GGGCCCT
EcoO65I 192 GGTGACC G^GT[ACGT]ACC
442 GGTTACC
HaeII 156 GGCGCT [GA]GCGC^[CT]
434 AGCGCC
Hin1I 3 GACGTC G[GA]^CG[CT]C
1096 GACGTC
HincII 355 GTCAAC GT[CT]^[GA]AC
1021 GTCAAC
HindII 355 GTCAAC GT[CT]^[GA]AC
1021 GTCAAC
HinfI 710 GAGTC G^A[ACGT]TC
1507 GATTC
Ksp632I 283 CTCTTC CTCTTC(1/4)
739 GAAGAG
MseI 212 TTAA T^TAA
1382 TTAA
MspA1I 181 CAGCTG C[AC]G^C[GT]G
438 CCGCGG
RsaI 678 GTAC GT^AC
1291 GTAC
SfaNI 92 GCATC GCATC(5/9)
1343 GCATC
SphI 53 GCATGC GCATG^C
666 GCATGC
TaqI 1100 TCGA T^CGA
1280 TCGA
Tru9I 212 TTAA T^TAA
1382 TTAA
Tsp45I 193 GTGAC ^GT[GC]AC
223 GTCAC
TthHB8I 1100 TCGA T^CGA
1280 TCGA
XmnI 1407 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1410 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI BalI BbvI
BcnI BsaJI BseRI BsgI BsiYI
BslI BsmFI BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PstI Sau3AI Sau96I
ScrFI SfcI StyI TseI TspRI