Restriction map for
pFN21AE4302
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspDI BspHI BspLU11I
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 214 CGCG CG^CG
AccIII 606 TCCGGA T^CCGGA
AcsI 457 GAATTC [GA]^AATT[CT]
AflIII 533 ACGTGT A^C[GA][CT]GT
ApoI 457 GAATTC [GA]^AATT[CT]
BalI 224 TGGCCA TGG^CCA
BamHI 609 GGATCC G^GATCC
BanI 361 GGTGCC G^G[CT][GA]CC
BcnI 671 CCGGG CC^[GC]GG
BfaI 421 CTAG C^TAG
BglI 364 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 443 CTGGAG CTGGAG(16/14)
BsaWI 606 TCCGGA [AT]^CCGG[AT]
BseAI 606 TCCGGA T^CCGGA
BsmI 599 GAATGC GAATGC(1/-1)
Bsp1407I 749 TGTACA T^GTACA
BspEI 606 TCCGGA T^CCGGA
BsrBI 88 GAGCGG CCGCTC(-3/-3)
BsrGI 749 TGTACA T^GTACA
BsrI 321 CCAGT ACTGG(1/-1)
BssSI 785 CTCGTG CACGAG(-5/-1)
BstEII 217 GGTAACC G^GT[ACGT]ACC
BstPI 217 GGTAACC G^GT[ACGT]ACC
BstUI 214 CGCG CG^CG
DdeI 835 CTCAG C^T[ACGT]AG
DraII 512 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 166 GACTGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 512 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 217 GGTAACC G^GT[ACGT]ACC
EcoRI 457 GAATTC G^AATTC
FauI 90 GCGGG CCCGC(4/6)
HincII 675 GTTGAC GT[CT]^[GA]AC
HindII 675 GTTGAC GT[CT]^[GA]AC
HindIII 803 AAGCTT A^AGCTT
HinfI 345 GAATC G^A[ACGT]TC
HphI 472 GGTGA GGTGA(8/7)
MaeI 421 CTAG C^TAG
MaeII 533 ACGT A^CGT
MluNI 224 TGGCCA TGG^CCA
MroI 606 TCCGGA T^CCGGA
MscI 224 TGGCCA TGG^CCA
MvnI 214 CGCG CG^CG
NciI 671 CCGGG CC^[GC]GG
NspI 117 GCATGC [GA]CATG^[CT]
SphI 117 GCATGC GCATG^C
SspBI 749 TGTACA T^GTACA
StuI 465 AGGCCT AGG^CCT
TaqI 86 TCGA T^CGA
TfiI 345 GAATC G^A[AT]TC
Tsp509I 458 AATT ^AATT
TthHB8I 86 TCGA T^CGA
XcmI 26 CCAAGGACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 610 GATCC GGATC(4/5)
609 GGATC
AvaII 174 GGTCC G^G[AT]CC
824 GGTCC
BglII 290 AGATCT A^GATCT
488 AGATCT
BsgI 112 GTGCAG GTGCAG(16/14)
541 GTGCAG
BspMI 40 GCAGGT ACCTGC(4/8)
108 GCAGGT
BstF5I 377 CATCC GGATG(2/0)
641 GGATG
EaeI 50 TGGCCG [CT]^GGCC[GA]
224 TGGCCA
FokI 377 CATCC GGATG(9/13)
641 GGATG
HaeII 78 AGCGCC [GA]GCGC^[CT]
329 GGCGCT
HapII 607 CCGG C^CGG
671 CCGG
HpaII 607 CCGG C^CGG
671 CCGG
MboII 777 GAAGA GAAGA(8/7)
293 TCTTC
MslI 377 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
711 CATGATGATG
MspI 607 CCGG C^CGG
671 CCGG
PstI 106 CTGCAG CTGCA^G
137 CTGCAG
PvuII 425 CAGCTG CAG^CTG
636 CAGCTG
SfcI 106 CTGCAG C^T[GA][CT]AG
137 CTGCAG
Tsp45I 473 GTGAC ^GT[GC]AC
658 GTCAC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsiYI BslI BssKI BstNI BstYI
Cac8I CfoI Cfr13I DpnII EcoRII
EcoT14I Fnu4HI HaeIII HhaI HinP1I
ItaI MaeIII MboI MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
TspRI XhoII