##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
AluI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HpaII       KasI        KpnI        
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SapI        Sau96I      SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          170  GTCGAC               GT^[AC][GT]AC
AccII         226  CGCG                 CG^CG
AciI          227  GCGG                 CCGC(-3/-1)
AfaI          157  GTAC                 GT^AC
AlwI            6  GATCC                GGATC(4/5)
BfaI           79  CTAG                 C^TAG
BsiYI         175  CCAGCCACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          175  CCAGCCACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         230  GGGAC                GGGAC(10/14)
BsrI          219  CCAGT                ACTGG(1/-1)
BssKI         301  CCTGG                ^CC[ACGT]GG
BstAPI        268  GCAGTGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        184  GGTTACC              G^GT[ACGT]ACC
BstF5I        143  GGATG                GGATG(2/0)
BstNI         301  CCTGG                CC^[AT]GG
BstPI         184  GGTTACC              G^GT[ACGT]ACC
BstUI         226  CGCG                 CG^CG
DpnII           6  GATC                 ^GATC
EarI          127  GAAGAG               CTCTTC(1/4)
EcoO65I       184  GGTTACC              G^GT[ACGT]ACC
EcoRII        301  CCTGG                ^CC[AT]GG
FauI          227  GCGGG                CCCGC(4/6)
FokI          143  GGATG                GGATG(9/13)
HincII        170  GTCGAC               GT[CT]^[GA]AC
HindII        170  GTCGAC               GT[CT]^[GA]AC
HinfI         132  GAATC                G^A[ACGT]TC
HphI            3  GGTGA                GGTGA(8/7)
Ksp632I       127  GAAGAG               CTCTTC(1/4)
MaeI           79  CTAG                 C^TAG
MaeIII        185  GTTAC                ^GT[ACGT]AC
MboI            6  GATC                 ^GATC
MseI           47  TTAA                 T^TAA
MvaI          301  CCTGG                CC^[AT]GG
MvnI          226  CGCG                 CG^CG
NdeII           6  GATC                 ^GATC
RsaI          157  GTAC                 GT^AC
SalI          170  GTCGAC               G^TCGAC
Sau3AI          6  GATC                 ^GATC
ScrFI         301  CCTGG                CC^[ACGT]GG
SexAI         300  ACCTGGT              A^CC[AT]GGT
SfcI           38  CTACAG               C^T[GA][CT]AG
TaqI          171  TCGA                 T^CGA
TfiI          132  GAATC                G^A[AT]TC
Tru9I          47  TTAA                 T^TAA
TspRI         269  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       171  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BbvI          118  GCAGC                GCAGC(8/12)
              333  GCAGC               
Cac8I         324  GCAAGC               GC[ACGT]^[ACGT]GC
              336  GCAAGC              
DdeI          200  CTCAG                C^T[ACGT]AG
              282  CTTAG               
Fnu4HI        118  GCAGC                GC^[ACGT]GC
              333  GCAGC               
ItaI          118  GCAGC                GC^[ACGT]GC
              333  GCAGC               
MnlI           29  CCTC                 CCTC(7/6)
              199  CCTC                
SfaNI         238  GATGC                GCATC(5/9)
               22  GCATC               
TseI          118  GCAGC                G^C[AT]GC
              333  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MboII       MwoI