Restriction map for
pFN21AE9417
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BanI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI Fnu4HI FokI FseI FspI
HaeII HapII HgaI Hin1I HindIII
HpaI HpaII ItaI KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
StuI StyI SwaI TseI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 133 GTCTAC GT^[AC][GT]AC
AflIII 582 ACGTGT A^C[GA][CT]GT
AseI 1089 ATTAAT AT^TAAT
AsnI 1089 ATTAAT AT^TAAT
AvaII 722 GGACC G^G[AT]CC
BalI 413 TGGCCA TGG^CCA
BamHI 488 GGATCC G^GATCC
BglII 335 AGATCT A^GATCT
BsiYI 58 CCCAGGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 58 CCCAGGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 349 GCATTC GAATGC(1/-1)
Bsp1407I 268 TGTACA T^GTACA
BspMI 559 ACCTGC ACCTGC(4/8)
BsrDI 68 GCAATG GCAATG(2/0)
BsrGI 268 TGTACA T^GTACA
BsrI 308 ACTGG ACTGG(1/-1)
CfoI 825 GCGC GCG^C
Cfr13I 722 GGACC G^G[ACGT]CC
DraI 280 TTTAAA TTT^AAA
DraIII 885 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 413 TGGCCA [CT]^GGCC[GA]
EcoRI 459 GAATTC G^AATTC
HhaI 825 GCGC GCG^C
HinP1I 825 GCGC G^CGC
HincII 541 GTCAAC GT[CT]^[GA]AC
HindII 541 GTCAAC GT[CT]^[GA]AC
MaeII 582 ACGT A^CGT
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
MwoI 563 GCTTTGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 760 CATATG CA^TATG
NspI 374 GCATGC [GA]CATG^[CT]
PleI 991 GAGTC GAGTC(4/5)
PshBI 1089 ATTAAT AT^TAAT
SapI 668 GCTCTTC GCTCTTC(1/4)
Sau96I 722 GGACC G^G[ACGT]CC
ScaI 738 AGTACT AGT^ACT
SfcI 135 CTACAG C^T[GA][CT]AG
SphI 374 GCATGC GCATG^C
SspBI 268 TGTACA T^GTACA
TaqI 432 TCGA T^CGA
TthHB8I 432 TCGA T^CGA
XcmI 306 CCACTGGCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 42 AAATTC [GA]^AATT[CT]
459 GAATTC
ApoI 42 AAATTC [GA]^AATT[CT]
459 GAATTC
Asp700I 946 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
957 GAAGTCCTTC
BbsI 591 GAAGAC GAAGAC(2/6)
187 GTCTTC
BclI 749 TGATCA T^GATCA
1062 TGATCA
BpuAI 591 GAAGAC GAAGAC(2/6)
187 GTCTTC
BsaJI 58 CCCAGG C^C[ACGT][ACGT]GG
818 CCCTGG
BsmFI 721 GGGAC GGGAC(10/14)
993 GTCCC
BstYI 335 AGATCT [GA]^GATC[CT]
488 GGATCC
FbaI 749 TGATCA T^GATCA
1062 TGATCA
HaeIII 311 GGCC GG^CC
414 GGCC
MaeIII 5 GTGAC ^GT[ACGT]AC
320 GTGAC
MflI 335 AGATCT [GA]^GATC[CT]
488 GGATCC
MslI 201 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
981 CATCAGAATG
NlaIV 488 GGATCC GG[ACGT]^[ACGT]CC
721 GGGACC
Tsp45I 5 GTGAC ^GT[GC]AC
320 GTGAC
XhoII 335 AGATCT [GA]^GATC[CT]
488 GGATCC
XmnI 946 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
957 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AfaI AluI AlwI BfaI
BsmAI BssKI BstNI Cac8I DdeI
DpnII EarI EcoRII HinfI HphI
Ksp632I MaeI MboI MboII MnlI
MseI MvaI NdeII NlaIII RsaI
Sau3AI ScrFI SspI TfiI Tru9I
Tsp509I TspRI