Restriction map for
pFN21AB6508
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccI AccII AccIII AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AspI
AviII AvrII BanII BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BlnI BsaAI BsaHI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoT14I EcoT22I FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI Tru9I Tsp509I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 299 GGTACC G^GTACC
AclI 217 AACGTT AA^CGTT
AlwNI 909 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 990 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 299 GGTACC G^GTACC
AspHI 278 GTGCTC G[AT]GC[AT]^C
AvaI 108 CCCGGG C^[CT]CG[GA]G
BamHI 113 GGATCC G^GATCC
BanI 299 GGTACC G^G[CT][GA]CC
BlpI 972 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 972 GCTGAGC GC^T[ACGT]AGC
BsaWI 1037 TCCGGT [AT]^CCGG[AT]
BseRI 262 CTCCTC GAGGAG(10/8)
BsiHKAI 278 GTGCTC G[AT]GC[AT]^C
BsmFI 102 GTCCC GGGAC(10/14)
BsoBI 108 CCCGGG C^[CT]CG[GA]G
Bsp1407I 196 TGTACA T^GTACA
BspLU11I 750 ACATGT A^CATGT
BsrDI 696 CATTGC GCAATG(2/0)
BsrGI 196 TGTACA T^GTACA
BssSI 1138 CACGAG CACGAG(-5/-1)
BstXI 503 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 972 GCTGAGC GC^T[ACGT]AGC
CfoI 397 GCGC GCG^C
EcoRV 439 GATATC GAT^ATC
FauI 920 CCCGC CCCGC(4/6)
HaeII 396 GGCGCT [GA]GCGC^[CT]
HhaI 397 GCGC GCG^C
HinP1I 397 GCGC G^CGC
HinfI 769 GAATC G^A[ACGT]TC
KpnI 299 GGTACC GGTAC^C
MspA1I 1006 CAGCTG C[AC]G^C[GT]G
NspI 750 ACATGT [GA]CATG^[CT]
PflMI 627 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 217 AACGTT AA^CGTT
PstI 131 CTGCAG CTGCA^G
PvuII 1006 CAGCTG CAG^CTG
SfcI 131 CTGCAG C^T[GA][CT]AG
SmaI 108 CCCGGG CCC^GGG
SmlI 1081 CTCAAG C^T[CT][GA]AG
SspBI 196 TGTACA T^GTACA
TaqI 670 TCGA T^CGA
TfiI 769 GAATC G^A[AT]TC
TthHB8I 670 TCGA T^CGA
Van91I 627 CCATTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
XmnI 990 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 274 ACGTGT A^C[GA][CT]GT
750 ACATGT
AvaII 39 GGACC G^G[AT]CC
101 GGTCC
BalI 512 TGGCCA TGG^CCA
1103 TGGCCA
BbsI 382 GTCTTC GAAGAC(2/6)
649 GTCTTC
BclI 182 TGATCA T^GATCA
1031 TGATCA
BglI 161 GCCTCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1019 GCCAGCCTGGC
BglII 188 AGATCT A^GATCT
467 AGATCT
BpmI 63 CTCCAG CTGGAG(16/14)
281 CTCCAG
BpuAI 382 GTCTTC GAAGAC(2/6)
649 GTCTTC
BsaBI 183 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
462 GATGAAGATC
BsaI 254 GAGACC GGTCTC(1/5)
1040 GGTCTC
BsmAI 254 GAGAC GTCTC(1/5)
1041 GTCTC
BsrBI 858 CCGCTC CCGCTC(-3/-3)
1141 GAGCGG
DsaI 734 CCGTGG C^C[GA][CT]GG
1100 CCGTGG
FbaI 182 TGATCA T^GATCA
1031 TGATCA
MamI 183 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
462 GATGAAGATC
MluNI 512 TGGCCA TGG^CCA
1103 TGGCCA
MscI 512 TGGCCA TGG^CCA
1103 TGGCCA
SfaNI 289 GATGC GCATC(5/9)
911 GCATC
StuI 159 AGGCCT AGG^CCT
446 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI XcmI XhoII