Restriction map for
pFN21AE1836
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeIII
MamI MfeI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 413 ACGTGT A^C[GA][CT]GT
AlwNI 521 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1039 GGGCCC GGGCC^C
BalI 359 TGGCCA TGG^CCA
BbsI 739 GAAGAC GAAGAC(2/6)
BbvCI 204 GCTGAGG CCTCAGC(-5/-2)
BglII 837 AGATCT A^GATCT
BlpI 239 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 239 GCTCAGC GC^T[ACGT]AGC
BpuAI 739 GAAGAC GAAGAC(2/6)
BsaI 892 GAGACC GGTCTC(1/5)
BsiYI 724 CCACTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 724 CCACTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 892 GAGAC GTCTC(1/5)
BsmFI 830 GTCCC GGGAC(10/14)
Bsp1407I 2 TGTACA T^GTACA
BspHI 32 TCATGA T^CATGA
BsrGI 2 TGTACA T^GTACA
BstBI 161 TTCGAA TT^CGAA
BstXI 362 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 239 GCTCAGC GC^T[ACGT]AGC
EaeI 359 TGGCCA [CT]^GGCC[GA]
EcoRV 501 GATATC GAT^ATC
FauI 587 CCCGC CCCGC(4/6)
MluNI 359 TGGCCA TGG^CCA
MscI 359 TGGCCA TGG^CCA
MseI 542 TTAA T^TAA
MwoI 869 GCTTTGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 161 TTCGAA TT^CGAA
PflMI 724 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 232 GACTC GAGTC(4/5)
RcaI 32 TCATGA T^CATGA
SfuI 161 TTCGAA TT^CGAA
SspBI 2 TGTACA T^GTACA
TaqI 162 TCGA T^CGA
TfiI 1056 GAATC G^A[AT]TC
Tru9I 542 TTAA T^TAA
TthHB8I 162 TCGA T^CGA
Van91I 724 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AlwI 614 GATCC GGATC(4/5)
624 GATCC
AvaII 79 GGACC G^G[AT]CC
254 GGTCC
BanII 60 GGGCTC G[GA]GC[CT]^C
1039 GGGCCC
BmyI 60 GGGCTC G[AGT]GC[ACT]^C
1039 GGGCCC
BsaJI 1034 CCCAGG C^C[ACGT][ACGT]GG
1035 CCAGGG
BsgI 55 GTGCAG GTGCAG(16/14)
330 GTGCAG
Bsp1286I 60 GGGCTC G[AGT]GC[ACT]^C
1039 GGGCCC
BspMI 895 ACCTGC ACCTGC(4/8)
931 ACCTGC
BsrDI 1003 CATTGC GCAATG(2/0)
437 GCAATG
BssKI 756 CCAGG ^CC[ACGT]GG
1035 CCAGG
BstNI 756 CCAGG CC^[AT]GG
1035 CCAGG
Cac8I 200 GCAGGC GC[ACGT]^[ACGT]GC
572 GCTTGC
DraII 1038 GGGGCCC [GA]G^G[ACGT]CC[CT]
1039 GGGCCCC
EarI 375 GAAGAG CTCTTC(1/4)
442 GAAGAG
EcoO109I 1038 GGGGCCC [GA]G^G[ACGT]CC[CT]
1039 GGGCCCC
EcoRII 756 CCAGG ^CC[AT]GG
1035 CCAGG
HinfI 232 GACTC G^A[ACGT]TC
1056 GAATC
HphI 21 GGTGA GGTGA(8/7)
921 TCACC
Ksp632I 375 GAAGAG CTCTTC(1/4)
442 GAAGAG
MspA1I 49 CAGCTG C[AC]G^C[GT]G
936 CAGCTG
MvaI 756 CCAGG CC^[AT]GG
1035 CCAGG
NspI 674 ACATGC [GA]CATG^[CT]
991 GCATGT
PstI 861 CTGCAG CTGCA^G
933 CTGCAG
PvuII 49 CAGCTG CAG^CTG
936 CAGCTG
ScrFI 756 CCAGG CC^[ACGT]GG
1035 CCAGG
SfcI 861 CTGCAG C^T[GA][CT]AG
933 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AfaI AluI Asp700I BbvI
BfaI BpmI BsrI BstF5I BstYI
Cfr13I DdeI DpnII Fnu4HI FokI
HaeIII ItaI MaeI MaeII MboI
MboII MflI MnlI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I SfaNI
TseI Tsp509I TspRI XhoII XmnI