Restriction map for
pFN21AE2083
##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AclI AflII AflIII AhdI AscI
AseI AsnI AspEI AspI AviII
AvrII BalI BamHI BbrPI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoT22I FseI FspI
HpaI MamI MfeI MluI MluNI
MscI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1599 GACGTC GACGT^C
Acc65I 2102 GGTACC G^GTACC
AccIII 1329 TCCGGA T^CCGGA
AgeI 101 ACCGGT A^CCGGT
Alw44I 1672 GTGCAC G^TGCAC
ApaI 1377 GGGCCC GGGCC^C
ApaLI 1672 GTGCAC G^TGCAC
Asp700I 1080 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2102 GGTACC G^GTACC
BbeI 1876 GGCGCC GGCGC^C
BcgI 1725 CGACAAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1502 TGATCA T^GATCA
BseAI 1329 TCCGGA T^CCGGA
BsiEI 2238 CGACCG CG[GA][CT]^CG
BspEI 1329 TCCGGA T^CCGGA
BsrBI 1522 GAGCGG CCGCTC(-3/-3)
BstEII 916 GGTGACC G^GT[ACGT]ACC
BstPI 916 GGTGACC G^GT[ACGT]ACC
EcoO65I 916 GGTGACC G^GT[ACGT]ACC
EcoRV 781 GATATC GAT^ATC
FbaI 1502 TGATCA T^GATCA
HgaI 1167 GACGC GACGC(5/10)
HincII 337 GTTGAC GT[CT]^[GA]AC
HindII 337 GTTGAC GT[CT]^[GA]AC
KasI 1876 GGCGCC G^GCGCC
KpnI 2102 GGTACC GGTAC^C
KspI 1367 CCGCGG CCGC^GG
MroI 1329 TCCGGA T^CCGGA
MslI 358 CATCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2131 GCCGGC GCC^GGC
NarI 1876 GGCGCC GG^CGCC
NgoMIV 2131 GCCGGC G^CCGGC
PinAI 101 ACCGGT A^CCGGT
SacI 1903 GAGCTC GAGCT^C
SacII 1367 CCGCGG CCGC^GG
ScaI 1475 AGTACT AGT^ACT
SexAI 1868 ACCAGGT A^CC[AT]GGT
SfoI 1876 GGCGCC GGC^GCC
SmlI 676 CTTGAG C^T[CT][GA]AG
StuI 1145 AGGCCT AGG^CCT
TfiI 403 GAATC G^A[AT]TC
XcmI 1385 CCATGTGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1080 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1657 GTAGAC GT^[AC][GT]AC
1828 GTCTAC
AcsI 226 AAATTC [GA]^AATT[CT]
301 AAATTC
AcyI 1599 GACGTC G[GA]^CG[CT]C
1876 GGCGCC
AlwI 480 GATCC GGATC(4/5)
2262 GATCC
AocI 328 CCTGAGG CC^T[ACGT]AGG
1933 CCTCAGG
Aor51HI 75 AGCGCT AGC^GCT
1754 AGCGCT
ApoI 226 AAATTC [GA]^AATT[CT]
301 AAATTC
AvaI 85 CCCGAG C^[CT]CG[GA]G
2141 CCCGAG
BanII 1377 GGGCCC G[GA]GC[CT]^C
1903 GAGCTC
BcnI 1092 CCGGG CC^[GC]GG
1668 CCGGG
BglI 2138 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2194 GCCAACAAGGC
BglII 986 AGATCT A^GATCT
1055 AGATCT
BsaHI 1599 GACGTC G[GA]^CG[CT]C
1876 GGCGCC
BsmBI 1601 CGTCTC CGTCTC(1/5)
1165 GAGACG
BsoBI 85 CCCGAG C^[CT]CG[GA]G
2141 CCCGAG
BstXI 627 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2072 CCAGGCGCATGG
Bsu36I 328 CCTGAGG CC^T[ACGT]AGG
1933 CCTCAGG
Eco47III 75 AGCGCT AGC^GCT
1754 AGCGCT
Eco81I 328 CCTGAGG CC^T[ACGT]AGG
1933 CCTCAGG
FauI 145 CCCGC CCCGC(4/6)
296 CCCGC
Hin1I 1599 GACGTC G[GA]^CG[CT]C
1876 GGCGCC
HindIII 673 AAGCTT A^AGCTT
907 AAGCTT
MaeII 1600 ACGT A^CGT
1613 ACGT
MseI 724 TTAA T^TAA
881 TTAA
NciI 1092 CCGGG CC^[GC]GG
1668 CCGGG
NcoI 2148 CCATGG C^CATGG
2231 CCATGG
PflMI 761 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1438 CCAGACCTTGG
PvuII 1014 CAGCTG CAG^CTG
1546 CAGCTG
Tru9I 724 TTAA T^TAA
881 TTAA
Van91I 761 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1438 CCAGACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BbsI BbvCI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII