Restriction map for
pFN21AE3323
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI Alw44I AlwNI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BcgI BclI BfaI BfrI
BlnI BsaAI BsaHI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BspEI
BspMI BssHII BssSI BstBI BstEII
BstPI BstXI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT14I EcoT22I
FbaI FseI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MluI
MluNI MroI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PstI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StyI SwaI Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 498 ACATGT A^C[GA][CT]GT
AgeI 1636 ACCGGT A^CCGGT
AocI 970 CCTCAGG CC^T[ACGT]AGG
AspHI 922 GAGCTC G[AT]GC[AT]^C
AvaII 2088 GGACC G^G[AT]CC
AviII 29 TGCGCA TGC^GCA
BglI 128 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 423 AGATCT A^GATCT
BpmI 905 CTGGAG CTGGAG(16/14)
BsaBI 508 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1636 ACCGGT [AT]^CCGG[AT]
BsiEI 1548 CGATCG CG[GA][CT]^CG
BsiHKAI 922 GAGCTC G[AT]GC[AT]^C
BspDI 2414 ATCGAT AT^CGAT
BspLU11I 498 ACATGT A^CATGT
BsrDI 1584 CATTGC GCAATG(2/0)
BsrFI 1636 ACCGGT [GA]^CCGG[CT]
Bst1107I 2191 GTATAC GTA^TAC
BstAPI 1332 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 423 AGATCT [GA]^GATC[CT]
BstZ17I 2191 GTATAC GTA^TAC
Bsu36I 970 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1636 ACCGGT [GA]^CCGG[CT]
ClaI 2414 ATCGAT AT^CGAT
DraIII 2713 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 970 CCTCAGG CC^T[ACGT]AGG
EcoNI 742 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2439 GAATTC G^AATTC
EcoRV 2150 GATATC GAT^ATC
FspI 29 TGCGCA TGC^GCA
HaeII 692 AGCGCC [GA]GCGC^[CT]
HgaI 1544 GACGC GACGC(5/10)
HincII 986 GTCAAC GT[CT]^[GA]AC
HindII 986 GTCAAC GT[CT]^[GA]AC
MamI 508 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 423 AGATCT [GA]^GATC[CT]
MslI 730 CATGATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 285 TCGCGA TCG^CGA
PinAI 1636 ACCGGT A^CCGGT
PpuMI 2087 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 1548 CGATCG CGAT^CG
SacI 922 GAGCTC GAGCT^C
SfcI 1691 CTACAG C^T[GA][CT]AG
SphI 1569 GCATGC GCATG^C
XhoII 423 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1616 GTAGAC GT^[AC][GT]AC
2191 GTATAC
AlwI 1081 GGATC GGATC(4/5)
2685 GGATC
BbsI 1296 GTCTTC GAAGAC(2/6)
2322 GTCTTC
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
859 GCTGAGG
BciVI 507 GGATAC GTATCC(6/5)
2580 GGATAC
BlpI 318 GCTCAGC GC^T[ACGT]AGC
924 GCTCAGC
Bpu1102I 318 GCTCAGC GC^T[ACGT]AGC
924 GCTCAGC
BpuAI 1296 GTCTTC GAAGAC(2/6)
2322 GTCTTC
BsmFI 2087 GGGAC GGGAC(10/14)
1120 GTCCC
BsmI 2166 GAATGC GAATGC(1/-1)
1055 GCATTC
Bsp1407I 1424 TGTACA T^GTACA
2568 TGTACA
BspHI 729 TCATGA T^CATGA
2510 TCATGA
BsrBI 58 GAGCGG CCGCTC(-3/-3)
1591 GAGCGG
BsrGI 1424 TGTACA T^GTACA
2568 TGTACA
CelII 318 GCTCAGC GC^T[ACGT]AGC
924 GCTCAGC
MaeII 26 ACGT A^CGT
1578 ACGT
MfeI 1781 CAATTG C^AATTG
2487 CAATTG
MspA1I 464 CAGCTG C[AC]G^C[GT]G
1420 CAGCTG
MunI 1781 CAATTG C^AATTG
2487 CAATTG
PflMI 592 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2498 CCAAACAATGG
PvuII 464 CAGCTG CAG^CTG
1420 CAGCTG
RcaI 729 TCATGA T^CATGA
2510 TCATGA
SapI 55 GAAGAGC GCTCTTC(1/4)
919 GAAGAGC
SspBI 1424 TGTACA T^GTACA
2568 TGTACA
StuI 280 AGGCCT AGG^CCT
1662 AGGCCT
Van91I 592 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2498 CCAAACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApaI ApoI AseI AsnI AvaI
BanI BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmaI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI