Restriction map for
pFN21AE3282
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BsiEI BsiWI BsmBI BsmI
Bsp1407I BspHI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII CpoI DraI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FseI HindIII HpaI KpnI MluI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 832 GTAGAC GT^[AC][GT]AC
AccIII 1813 TCCGGA T^CCGGA
AcyI 42 GGCGCC G[GA]^CG[CT]C
AflIII 755 ACATGT A^C[GA][CT]GT
Aor51HI 1157 AGCGCT AGC^GCT
AspI 1791 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 628 TGCGCA TGC^GCA
BbeI 42 GGCGCC GGCGC^C
BbrPI 241 CACGTG CAC^GTG
BcgI 1034 CGAGCGTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1085 TGATCA T^GATCA
BglI 428 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1995 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 42 GGCGCC G[GA]^CG[CT]C
BsaI 1857 GGTCTC GGTCTC(1/5)
BseAI 1813 TCCGGA T^CCGGA
BspDI 730 ATCGAT AT^CGAT
BspEI 1813 TCCGGA T^CCGGA
BspLU11I 755 ACATGT A^CATGT
BsrDI 1931 GCAATG GCAATG(2/0)
BssSI 1277 CACGAG CACGAG(-5/-1)
ClaI 730 ATCGAT AT^CGAT
DrdI 301 GACACCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1157 AGCGCT AGC^GCT
EcoNI 1440 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1288 GAATTC G^AATTC
FbaI 1085 TGATCA T^GATCA
FspI 628 TGCGCA TGC^GCA
Hin1I 42 GGCGCC G[GA]^CG[CT]C
HincII 768 GTTGAC GT[CT]^[GA]AC
HindII 768 GTTGAC GT[CT]^[GA]AC
KasI 42 GGCGCC G^GCGCC
KspI 83 CCGCGG CCGC^GG
MamI 1995 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1813 TCCGGA T^CCGGA
MspA1I 83 CCGCGG C[AC]G^C[GT]G
NarI 42 GGCGCC GG^CGCC
NcoI 1550 CCATGG C^CATGG
NruI 1698 TCGCGA TCG^CGA
PflFI 1791 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 241 CACGTG CAC^GTG
PmlI 241 CACGTG CAC^GTG
PpuMI 1579 AGGACCC [GA]G^G[AT]CC[CT]
SacII 83 CCGCGG CCGC^GG
SexAI 410 ACCTGGT A^CC[AT]GGT
SfoI 42 GGCGCC GGC^GCC
SmaI 1800 CCCGGG CCC^GGG
Tth111I 1791 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1940 CCATGCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1800 CCCGGG C^CCGGG
XmaI 1800 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 819 GTAC GT^AC
1230 GTAC
AocI 807 CCTGAGG CC^T[ACGT]AGG
1400 CCTCAGG
ApaI 1235 GGGCCC GGGCC^C
1621 GGGCCC
AspHI 523 GAGCAC G[AT]GC[AT]^C
1096 GTGCTC
BalI 263 TGGCCA TGG^CCA
1124 TGGCCA
BanI 42 GGCGCC G^G[CT][GA]CC
874 GGTGCC
BbsI 586 GAAGAC GAAGAC(2/6)
1651 GAAGAC
BfaI 580 CTAG C^TAG
2089 CTAG
BpuAI 586 GAAGAC GAAGAC(2/6)
1651 GAAGAC
BsaAI 241 CACGTG [CT]AC^GT[GA]
1708 CACGTA
BseRI 475 GAGGAG GAGGAG(10/8)
1897 GAGGAG
BsiHKAI 523 GAGCAC G[AT]GC[AT]^C
1096 GTGCTC
BsrBI 123 GAGCGG CCGCTC(-3/-3)
139 GAGCGG
BstXI 1361 CCAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1529 CCAATTGTGTGG
Bsu36I 807 CCTGAGG CC^T[ACGT]AGG
1400 CCTCAGG
DraIII 663 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
665 CACAGTGTG
DsaI 83 CCGCGG C^C[GA][CT]GG
1550 CCATGG
EarI 285 GAAGAG CTCTTC(1/4)
918 GAAGAG
Eco81I 807 CCTGAGG CC^T[ACGT]AGG
1400 CCTCAGG
Ksp632I 285 GAAGAG CTCTTC(1/4)
918 GAAGAG
MaeI 580 CTAG C^TAG
2089 CTAG
MfeI 1530 CAATTG C^AATTG
1962 CAATTG
MluNI 263 TGGCCA TGG^CCA
1124 TGGCCA
MscI 263 TGGCCA TGG^CCA
1124 TGGCCA
MslI 744 CATAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1560 CATCCCCGTG
MunI 1530 CAATTG C^AATTG
1962 CAATTG
NaeI 3 GCCGGC GCC^GGC
36 GCCGGC
NgoMIV 3 GCCGGC G^CCGGC
36 GCCGGC
NspI 755 ACATGT [GA]CATG^[CT]
1820 GCATGT
PflMI 822 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCACATCCTGG
RsaI 819 GTAC GT^AC
1230 GTAC
SspI 928 AATATT AAT^ATT
1504 AATATT
StuI 1256 AGGCCT AGG^CCT
1457 AGGCCT
Tsp45I 310 GTCAC ^GT[GC]AC
994 GTCAC
Van91I 822 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCACATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AvaI AvaII BamHI
BanII BbvI BcnI BglII BmyI
BpmI BsaJI BsaWI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII