##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
ItaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TseI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI           63  GCGG                 CCGC(-3/-1)
AvaII         175  GGACC                G^G[AT]CC
BfaI          188  CTAG                 C^TAG
BsaJI         317  CCCAGG               C^C[ACGT][ACGT]GG
BseRI          27  CTCCTC               GAGGAG(10/8)
BsmAI          49  GTCTC                GTCTC(1/5)
BsmBI          48  CGTCTC               CGTCTC(1/5)
BssKI         318  CCAGG                ^CC[ACGT]GG
BstNI         318  CCAGG                CC^[AT]GG
BstYI         288  AGATCC               [GA]^GATC[CT]
Cfr13I        175  GGACC                G^G[ACGT]CC
DraII         174  AGGACCT              [GA]G^G[ACGT]CC[CT]
EarI           16  CTCTTC               CTCTTC(1/4)
EcoO109I      174  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII        318  CCAGG                ^CC[AT]GG
HaeIII        321  GGCC                 GG^CC
HinfI           3  GATTC                G^A[ACGT]TC
Ksp632I        16  CTCTTC               CTCTTC(1/4)
MaeI          188  CTAG                 C^TAG
MaeIII         79  GTTAC                ^GT[ACGT]AC
MflI          288  AGATCC               [GA]^GATC[CT]
MvaI          318  CCAGG                CC^[AT]GG
MwoI          222  GCTTTTTAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        258  CATG                 CATG^
PpuMI         174  AGGACCT              [GA]G^G[AT]CC[CT]
SapI           15  GCTCTTC              GCTCTTC(1/4)
Sau96I        175  GGACC                G^G[ACGT]CC
ScrFI         318  CCAGG                CC^[ACGT]GG
SfaNI         184  GATGC                GCATC(5/9)
SmlI          232  CTCAAG               C^T[CT][GA]AG
TaqI          308  TCGA                 T^CGA
TfiI            3  GATTC                G^A[AT]TC
TspRI          86  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       308  TCGA                 T^CGA
XhoII         288  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          280  AAATTT               [GA]^AATT[CT]
              295  AAATTT              
AlwI          109  GATCC                GGATC(4/5)
              289  GATCC               
ApoI          280  AAATTT               [GA]^AATT[CT]
              295  AAATTT              
BpmI          170  CTGGAG               CTGGAG(16/14)
              211  CTGGAG              
DdeI           51  CTCAG                C^T[ACGT]AG
              155  CTAAG               
DpnII         109  GATC                 ^GATC
              289  GATC                
MboI          109  GATC                 ^GATC
              289  GATC                
MseI           95  TTAA                 T^TAA
              227  TTAA                
NdeII         109  GATC                 ^GATC
              289  GATC                
Sau3AI        109  GATC                 ^GATC
              289  GATC                
Tru9I          95  TTAA                 T^TAA
              227  TTAA                
Tsp509I       281  AATT                 ^AATT
              296  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AluI        MboII       MnlI