Restriction map for
pFN21AE3755
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII CfoI Cfr10I CpoI
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 487 GTATAC GT^[AC][GT]AC
AcsI 133 GAATTT [GA]^AATT[CT]
AfaI 525 GTAC GT^AC
AlwNI 367 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 133 GAATTT [GA]^AATT[CT]
BamHI 554 GGATCC G^GATCC
BanI 757 GGCACC G^G[CT][GA]CC
BcnI 753 CCGGG CC^[GC]GG
BglII 228 AGATCT A^GATCT
BmyI 756 GGGCAC G[AGT]GC[ACT]^C
BseRI 650 CTCCTC GAGGAG(10/8)
BsiEI 108 CGATCG CG[GA][CT]^CG
Bsp1286I 756 GGGCAC G[AGT]GC[ACT]^C
BspDI 106 ATCGAT AT^CGAT
Bst1107I 487 GTATAC GTA^TAC
BstF5I 750 CATCC GGATG(2/0)
BstZ17I 487 GTATAC GTA^TAC
ClaI 106 ATCGAT AT^CGAT
DraI 136 TTTAAA TTT^AAA
DrdI 880 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 975 CCCGC CCCGC(4/6)
FokI 750 CATCC GGATG(9/13)
HaeIII 295 GGCC GG^CC
HincII 582 GTTGAC GT[CT]^[GA]AC
HindII 582 GTTGAC GT[CT]^[GA]AC
MslI 340 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 927 CAGCTG C[AC]G^C[GT]G
NciI 753 CCGGG CC^[GC]GG
PstI 232 CTGCAG CTGCA^G
PvuI 108 CGATCG CGAT^CG
PvuII 927 CAGCTG CAG^CTG
RsaI 525 GTAC GT^AC
SfcI 232 CTGCAG C^T[GA][CT]AG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccIII 71 TCCGGA T^CCGGA
462 TCCGGA
BbvI 234 GCAGC GCAGC(8/12)
722 GCAGC
BfaI 226 CTAG C^TAG
304 CTAG
BpmI 727 CTCCAG CTGGAG(16/14)
451 CTGGAG
BsaWI 71 TCCGGA [AT]^CCGG[AT]
462 TCCGGA
BseAI 71 TCCGGA T^CCGGA
462 TCCGGA
BsgI 113 GTGCAG GTGCAG(16/14)
505 GTGCAG
BspEI 71 TCCGGA T^CCGGA
462 TCCGGA
DsaI 287 CCATGG C^C[GA][CT]GG
324 CCATGG
EcoT14I 287 CCATGG C^C[AT][AT]GG
324 CCATGG
MaeI 226 CTAG C^TAG
304 CTAG
MaeIII 436 GTTAC ^GT[ACGT]AC
702 GTTAC
MroI 71 TCCGGA T^CCGGA
462 TCCGGA
MseI 137 TTAA T^TAA
764 TTAA
NcoI 287 CCATGG C^CATGG
324 CCATGG
NlaIV 554 GGATCC GG[ACGT]^[ACGT]CC
757 GGCACC
PflMI 324 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCAGGACTTGG
PleI 242 GACTC GAGTC(4/5)
676 GAGTC
SfaNI 200 GCATC GCATC(5/9)
743 GCATC
StyI 287 CCATGG C^C[AT][AT]GG
324 CCATGG
TaqI 107 TCGA T^CGA
855 TCGA
TfiI 534 GAATC G^A[AT]TC
630 GATTC
Tru9I 137 TTAA T^TAA
764 TTAA
TseI 234 GCAGC G^C[AT]GC
722 GCAGC
TthHB8I 107 TCGA T^CGA
855 TCGA
Van91I 324 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
729 CCAGGACTTGG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI AvaII BbsI
BpuAI BsaJI BsiYI BslI BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII Fnu4HI HapII HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MflI MnlI MspI MvaI MwoI
NdeII NlaIII PpuMI Sau3AI Sau96I
ScrFI Tsp509I TspRI XhoII