Restriction map for
pFN21AE2599
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraIII EarI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 392 GGTACC G^GTACC
AflIII 336 ACACGT A^C[GA][CT]GT
AhdI 1091 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1138 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 888 CCTCAGG CC^T[ACGT]AGG
Aor51HI 902 AGCGCT AGC^GCT
Asp718I 392 GGTACC G^GTACC
AspEI 1091 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 658 GAGCTC G[AT]GC[AT]^C
AvaI 171 CCCGGG C^[CT]CG[GA]G
BalI 587 TGGCCA TGG^CCA
BglI 90 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 658 GAGCTC G[AT]GC[AT]^C
BsoBI 171 CCCGGG C^[CT]CG[GA]G
BspMI 6 ACCTGC ACCTGC(4/8)
BsrBI 1207 CCGCTC CCGCTC(-3/-3)
BsrDI 540 CATTGC GCAATG(2/0)
BsrI 981 ACTGG ACTGG(1/-1)
BstYI 930 GGATCT [GA]^GATC[CT]
Bsu36I 888 CCTCAGG CC^T[ACGT]AGG
DraI 969 TTTAAA TTT^AAA
DrdI 355 GACCAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 138 CGGCCG C^GGCCG
Eam1105I 1091 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 138 CGGCCG C^GGCCG
Eco47III 902 AGCGCT AGC^GCT
Eco52I 138 CGGCCG C^GGCCG
Eco81I 888 CCTCAGG CC^T[ACGT]AGG
HgaI 1168 GACGC GACGC(5/10)
KpnI 392 GGTACC GGTAC^C
MaeIII 610 GTGAC ^GT[ACGT]AC
MflI 930 GGATCT [GA]^GATC[CT]
MluNI 587 TGGCCA TGG^CCA
MscI 587 TGGCCA TGG^CCA
MseI 970 TTAA T^TAA
MslI 480 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 95 GCCGGC GCC^GGC
NgoMIV 95 GCCGGC G^CCGGC
NlaIII 74 CATG CATG^
PleI 954 GACTC GAGTC(4/5)
PpuMI 467 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 1138 CAGCTG CAG^CTG
SacI 658 GAGCTC GAGCT^C
SmaI 171 CCCGGG CCC^GGG
TaqI 899 TCGA T^CGA
TfiI 692 GATTC G^A[AT]TC
Tru9I 970 TTAA T^TAA
Tsp45I 610 GTGAC ^GT[GC]AC
TthHB8I 899 TCGA T^CGA
XhoII 930 GGATCT [GA]^GATC[CT]
XmaCI 171 CCCGGG C^CCGGG
XmaI 171 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BanII 283 GAGCCC G[GA]GC[CT]^C
658 GAGCTC
BbeI 448 GGCGCC GGCGC^C
853 GGCGCC
BsiEI 134 CGGTCG CG[GA][CT]^CG
138 CGGCCG
BsrFI 95 GCCGGC [GA]^CCGG[CT]
1120 ACCGGC
BstF5I 462 CATCC GGATG(2/0)
834 CATCC
Cfr10I 95 GCCGGC [GA]^CCGG[CT]
1120 ACCGGC
EcoNI 307 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
884 CCTTCCTCAGG
FokI 462 CATCC GGATG(9/13)
834 CATCC
HinfI 692 GATTC G^A[ACGT]TC
954 GACTC
HphI 486 GGTGA GGTGA(8/7)
767 TCACC
KasI 448 GGCGCC G^GCGCC
853 GGCGCC
MaeII 338 ACGT A^CGT
785 ACGT
MspA1I 109 CAGCGG C[AC]G^C[GT]G
1138 CAGCTG
NarI 448 GGCGCC GG^CGCC
853 GGCGCC
SfaNI 159 GCATC GCATC(5/9)
842 GCATC
SfoI 448 GGCGCC GGC^GCC
853 GGCGCC
SmlI 661 CTCAAG C^T[CT][GA]AG
956 CTCAAG
XcmI 396 CCAGGGTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
740 CCAGACCCGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BanI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HpaII ItaI MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI TspRI