Restriction map for
pFN21AB6201
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BanI BbeI BbrPI
BfrI BglI BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TfiI
XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1827 CGCG CG^CG
AflIII 1329 ACGTGT A^C[GA][CT]GT
Alw44I 253 GTGCAC G^TGCAC
Aor51HI 1360 AGCGCT AGC^GCT
ApaI 373 GGGCCC GGGCC^C
ApaLI 253 GTGCAC G^TGCAC
AspHI 253 GTGCAC G[AT]GC[AT]^C
AspI 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1232 GGACC G^G[AT]CC
AviII 1340 TGCGCA TGC^GCA
BbvCI 1849 GCTGAGG CCTCAGC(-5/-2)
BcgI 1839 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 410 GTATCC GTATCC(6/5)
BclI 536 TGATCA T^GATCA
BcnI 377 CCCGG CC^[GC]GG
BglII 14 AGATCT A^GATCT
BlpI 297 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC
BsaAI 1328 TACGTG [CT]AC^GT[GA]
BsiHKAI 253 GTGCAC G[AT]GC[AT]^C
BsiWI 883 CGTACG C^GTACG
BsmBI 1835 GAGACG CGTCTC(1/5)
BsmI 1195 GAATGC GAATGC(1/-1)
BspHI 1011 TCATGA T^CATGA
BsrDI 1308 CATTGC GCAATG(2/0)
BstUI 1827 CGCG CG^CG
BstYI 14 AGATCT [GA]^GATC[CT]
CelII 297 GCTGAGC GC^T[ACGT]AGC
DraI 1024 TTTAAA TTT^AAA
Eco47III 1360 AGCGCT AGC^GCT
EcoNI 78 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 536 TGATCA T^GATCA
FspI 1340 TGCGCA TGC^GCA
HgaI 176 GCGTC GACGC(5/10)
HincII 910 GTTAAC GT[CT]^[GA]AC
HindII 910 GTTAAC GT[CT]^[GA]AC
HinfI 1928 GAGTC G^A[ACGT]TC
HpaI 910 GTTAAC GTT^AAC
MflI 14 AGATCT [GA]^GATC[CT]
MvnI 1827 CGCG CG^CG
NciI 377 CCCGG CC^[GC]GG
PflFI 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 567 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1928 GAGTC GAGTC(4/5)
PvuII 695 CAGCTG CAG^CTG
RcaI 1011 TCATGA T^CATGA
SapI 550 GAAGAGC GCTCTTC(1/4)
SphI 1350 GCATGC GCATG^C
StuI 75 AGGCCT AGG^CCT
Tth111I 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 567 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 14 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 660 AAATTC [GA]^AATT[CT]
1617 AAATTT
ApoI 660 AAATTC [GA]^AATT[CT]
1617 AAATTT
AvaI 55 CTCGAG C^[CT]CG[GA]G
544 CTCGAG
BalI 401 TGGCCA TGG^CCA
485 TGGCCA
BanII 126 GAGCCC G[GA]GC[CT]^C
373 GGGCCC
BbsI 493 GAAGAC GAAGAC(2/6)
1396 GTCTTC
BpmI 181 CTGGAG CTGGAG(16/14)
1526 CTGGAG
BpuAI 493 GAAGAC GAAGAC(2/6)
1396 GTCTTC
BsmAI 1139 GAGAC GTCTC(1/5)
1835 GAGAC
BsoBI 55 CTCGAG C^[CT]CG[GA]G
544 CTCGAG
BsrBI 1505 CCGCTC CCGCTC(-3/-3)
1227 GAGCGG
EaeI 401 TGGCCA [CT]^GGCC[GA]
485 TGGCCA
HapII 67 CCGG C^CGG
378 CCGG
HpaII 67 CCGG C^CGG
378 CCGG
MaeII 1329 ACGT A^CGT
1494 ACGT
MluNI 401 TGGCCA TGG^CCA
485 TGGCCA
MscI 401 TGGCCA TGG^CCA
485 TGGCCA
MspA1I 695 CAGCTG C[AC]G^C[GT]G
1668 CCGCTG
MspI 67 CCGG C^CGG
378 CCGG
NspI 785 GCATGT [GA]CATG^[CT]
1350 GCATGC
PaeR7I 55 CTCGAG C^TCGAG
544 CTCGAG
TaqI 56 TCGA T^CGA
545 TCGA
TthHB8I 56 TCGA T^CGA
545 TCGA
XbaI 465 TCTAGA T^CTAGA
799 TCTAGA
XhoI 55 CTCGAG C^TCGAG
544 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I BbvI BfaI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI Tru9I TseI Tsp45I
Tsp509I TspRI XmnI