Restriction map for
pFN21AE2529
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssSI Bst1107I BstAPI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DpnII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I MaeII MamI MboI MboII
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfcI SfiI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwNI 259 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 324 ATTAAT AT^TAAT
AsnI 324 ATTAAT AT^TAAT
AvaI 47 CCCGGG C^[CT]CG[GA]G
BmyI 514 GTGCCC G[AGT]GC[ACT]^C
BpmI 560 CTCCAG CTGGAG(16/14)
BseRI 235 GAGGAG GAGGAG(10/8)
BsgI 228 CTGCAC GTGCAG(16/14)
BsmAI 508 GTCTC GTCTC(1/5)
BsoBI 47 CCCGGG C^[CT]CG[GA]G
Bsp1286I 514 GTGCCC G[AGT]GC[ACT]^C
BsrDI 305 CATTGC GCAATG(2/0)
BsrI 484 CCAGT ACTGG(1/-1)
BssHII 160 GCGCGC G^CGCGC
BstBI 536 TTCGAA TT^CGAA
DraII 185 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 185 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 332 CCTTGG C^C[AT][AT]GG
KspI 174 CCGCGG CCGC^GG
MaeIII 255 GTCAC ^GT[ACGT]AC
MslI 348 CATGGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 371 ACATGC [GA]CATG^[CT]
NspV 536 TTCGAA TT^CGAA
PleI 406 GAGTC GAGTC(4/5)
PpuMI 185 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 324 ATTAAT AT^TAAT
SacII 174 CCGCGG CCGC^GG
SfuI 536 TTCGAA TT^CGAA
SmaI 47 CCCGGG CCC^GGG
StyI 332 CCTTGG C^C[AT][AT]GG
TaqI 537 TCGA T^CGA
Tsp45I 255 GTCAC ^GT[GC]AC
Tsp509I 327 AATT ^AATT
TthHB8I 537 TCGA T^CGA
XcmI 65 CCACCTGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 47 CCCGGG C^CCGGG
XmaI 47 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 415 GTAC GT^AC
443 GTAC
BanI 43 GGCGCC G^G[CT][GA]CC
79 GGCGCC
BbeI 43 GGCGCC GGCGC^C
79 GGCGCC
BfaI 136 CTAG C^TAG
505 CTAG
DraI 400 TTTAAA TTT^AAA
553 TTTAAA
DsaI 174 CCGCGG C^C[GA][CT]GG
200 CCACGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
177 CGGCCA
HaeII 43 GGCGCC [GA]GCGC^[CT]
79 GGCGCC
HgaI 139 GACGC GACGC(5/10)
221 GCGTC
KasI 43 GGCGCC G^GCGCC
79 GGCGCC
MaeI 136 CTAG C^TAG
505 CTAG
NarI 43 GGCGCC GG^CGCC
79 GGCGCC
NlaIII 348 CATG CATG^
372 CATG
PvuII 225 CAGCTG CAG^CTG
563 CAGCTG
RsaI 415 GTAC GT^AC
443 GTAC
SfoI 43 GGCGCC GGC^GCC
79 GGCGCC
TfiI 317 GAATC G^A[AT]TC
491 GAATC
TspRI 291 CACTG CA[GC]TG[ACGT][ACGT]^
485 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BbvI BcnI BsaHI BsaJI BsiYI
BslI BsmFI BssKI BstNI BstUI
Cac8I CfoI Cfr13I EcoRII FauI
Fnu4HI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
Tru9I TseI