Restriction map for
pFN21AE6464
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BbrPI
BbsI BciVI BclI BfrI BlnI
BpuAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 475 GGTACC G^GTACC
Asp718I 475 GGTACC G^GTACC
BalI 1563 TGGCCA TGG^CCA
BamHI 796 GGATCC G^GATCC
BfaI 901 CTAG C^TAG
BlpI 123 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTAAGC GC^T[ACGT]AGC
BsaAI 364 TACGTG [CT]AC^GT[GA]
BsaI 1142 GAGACC GGTCTC(1/5)
BsmBI 1542 GAGACG CGTCTC(1/5)
BsmFI 408 GTCCC GGGAC(10/14)
BspLU11I 497 ACATGT A^CATGT
BspMI 519 ACCTGC ACCTGC(4/8)
BsrBI 1202 CCGCTC CCGCTC(-3/-3)
BstAPI 1212 GCATGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 123 GCTAAGC GC^T[ACGT]AGC
KpnI 475 GGTACC GGTAC^C
MaeI 901 CTAG C^TAG
MaeIII 1288 GTGAC ^GT[ACGT]AC
MluNI 1563 TGGCCA TGG^CCA
MscI 1563 TGGCCA TGG^CCA
NheI 900 GCTAGC G^CTAGC
PflMI 1506 CCAGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 587 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1452 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1173 GAGCTC GAGCT^C
SmaI 630 CCCGGG CCC^GGG
SphI 1212 GCATGC GCATG^C
Tsp45I 1288 GTGAC ^GT[GC]AC
Van91I 1506 CCAGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 630 CCCGGG C^CCGGG
XmaI 630 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 313 CGCG CG^CG
673 CGCG
AflIII 365 ACGTGT A^C[GA][CT]GT
497 ACATGT
AlwI 797 GATCC GGATC(4/5)
796 GGATC
ApaI 627 GGGCCC GGGCC^C
1189 GGGCCC
AspHI 715 GTGCTC G[AT]GC[AT]^C
1173 GAGCTC
BcgI 1605 CGAGATCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
938 GCATAGGCCTCG
BglI 805 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
913 GCCCTCATGGC
BsgI 515 CTGCAC GTGCAG(16/14)
878 GTGCAG
BsiHKAI 715 GTGCTC G[AT]GC[AT]^C
1173 GAGCTC
BstUI 313 CGCG CG^CG
673 CGCG
BstXI 860 CCATGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1285 CCAGTGACCTGG
EaeI 1370 CGGCCA [CT]^GGCC[GA]
1563 TGGCCA
EcoNI 411 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1038 CCTGCTGCAGG
FauI 1201 CCCGC CCCGC(4/6)
393 GCGGG
MaeII 365 ACGT A^CGT
746 ACGT
MslI 426 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1235 CACTGCTGTG
MspA1I 391 CAGCGG C[AC]G^C[GT]G
1454 CCGCTG
MvnI 313 CGCG CG^CG
673 CGCG
PstI 106 CTGCAG CTGCA^G
1042 CTGCAG
SapI 1334 GAAGAGC GCTCTTC(1/4)
717 GCTCTTC
SfcI 106 CTGCAG C^T[GA][CT]AG
1042 CTGCAG
StuI 942 AGGCCT AGG^CCT
1017 AGGCCT
TaqI 1400 TCGA T^CGA
1604 TCGA
TthHB8I 1400 TCGA T^CGA
1604 TCGA
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BglII BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI XcmI
XhoII