Restriction map for
pFN21AE5417
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I MaeIII
MamI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 29 CGCG CG^CG
AcyI 16 GACGCC G[GA]^CG[CT]C
AfaI 264 GTAC GT^AC
AlwNI 182 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 650 CTCGAG C^[CT]CG[GA]G
BanI 679 GGCACC G^G[CT][GA]CC
BbsI 253 GTCTTC GAAGAC(2/6)
BfaI 871 CTAG C^TAG
BglI 587 GCCGGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 757 GCTGAGC GC^T[ACGT]AGC
BpmI 364 CTGGAG CTGGAG(16/14)
Bpu1102I 757 GCTGAGC GC^T[ACGT]AGC
BpuAI 253 GTCTTC GAAGAC(2/6)
BsaHI 16 GACGCC G[GA]^CG[CT]C
BsgI 139 GTGCAG GTGCAG(16/14)
BsiEI 534 CGACCG CG[GA][CT]^CG
BsoBI 650 CTCGAG C^[CT]CG[GA]G
BspMI 135 GCAGGT ACCTGC(4/8)
BsrBI 156 CCGCTC CCGCTC(-3/-3)
BstAPI 765 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 29 CGCG CG^CG
BstXI 191 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 757 GCTGAGC GC^T[ACGT]AGC
CfoI 80 GCGC GCG^C
DdeI 758 CTGAG C^T[ACGT]AG
HhaI 80 GCGC GCG^C
Hin1I 16 GACGCC G[GA]^CG[CT]C
HinP1I 80 GCGC G^CGC
KspI 28 CCGCGG CCGC^GG
MaeI 871 CTAG C^TAG
MaeII 296 ACGT A^CGT
MseI 719 TTAA T^TAA
MvnI 29 CGCG CG^CG
NaeI 547 GCCGGC GCC^GGC
NcoI 197 CCATGG C^CATGG
NgoMIV 547 GCCGGC G^CCGGC
NspI 770 ACATGC [GA]CATG^[CT]
PaeR7I 650 CTCGAG C^TCGAG
PstI 220 CTGCAG CTGCA^G
RsaI 264 GTAC GT^AC
SacII 28 CCGCGG CCGC^GG
SfcI 220 CTGCAG C^T[GA][CT]AG
SmlI 650 CTCGAG C^T[CT][GA]AG
Tru9I 719 TTAA T^TAA
XhoI 650 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 270 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
577 GAACCAATTC
BmyI 467 GTGCCC G[AGT]GC[ACT]^C
678 GGGCAC
BsmAI 302 GAGAC GTCTC(1/5)
934 GAGAC
Bsp1286I 467 GTGCCC G[AGT]GC[ACT]^C
678 GGGCAC
BsrFI 131 ACCGGC [GA]^CCGG[CT]
547 GCCGGC
Cfr10I 131 ACCGGC [GA]^CCGG[CT]
547 GCCGGC
DsaI 28 CCGCGG C^C[GA][CT]GG
197 CCATGG
EaeI 444 TGGCCG [CT]^GGCC[GA]
833 CGGCCA
EcoNI 363 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
804 CCTCATCGAGG
EcoT14I 109 CCTTGG C^C[AT][AT]GG
197 CCATGG
HgaI 16 GACGC GACGC(5/10)
325 GACGC
HinfI 625 GAATC G^A[ACGT]TC
692 GAATC
MslI 192 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
633 CACCTTTGTG
StyI 109 CCTTGG C^C[AT][AT]GG
197 CCATGG
TfiI 625 GAATC G^A[AT]TC
692 GAATC
Tsp509I 582 AATT ^AATT
878 AATT
XcmI 632 CCACCTTTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
656 CCAACAAAGCCCTGG
XmnI 270 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
577 GAACCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BsaJI BseRI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII ItaI MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI PvuII Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TspRI
TthHB8I XhoII