Restriction map for
pFN21AB7040
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FokI FseI
HaeII HapII HgaI Hin1I HindIII
HpaI HpaII KasI KpnI KspI
MaeIII MamI MfeI MluI MroI
MspI MunI MvnI MwoI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 563 GCGG CCGC(-3/-1)
Alw44I 539 GTGCAC G^TGCAC
ApaLI 539 GTGCAC G^TGCAC
AviII 720 TGCGCA TGC^GCA
BalI 1221 TGGCCA TGG^CCA
BanI 1167 GGCACC G^G[CT][GA]CC
BanII 775 GAGCTC G[GA]GC[CT]^C
BbvI 55 GCAGC GCAGC(8/12)
BclI 222 TGATCA T^GATCA
BsaI 341 GAGACC GGTCTC(1/5)
BsaJI 1189 CCATGG C^C[ACGT][ACGT]GG
BseRI 34 CTCCTC GAGGAG(10/8)
BsiEI 1365 CGACCG CG[GA][CT]^CG
BsmBI 531 CGTCTC CGTCTC(1/5)
BsmI 261 GAATGC GAATGC(1/-1)
BssKI 1164 CCAGG ^CC[ACGT]GG
BstNI 1164 CCAGG CC^[AT]GG
CfoI 721 GCGC GCG^C
Cfr13I 132 GGGCC G^G[ACGT]CC
DsaI 1189 CCATGG C^C[GA][CT]GG
EcoRII 1164 CCAGG ^CC[AT]GG
EcoT14I 1189 CCATGG C^C[AT][AT]GG
FbaI 222 TGATCA T^GATCA
Fnu4HI 55 GCAGC GC^[ACGT]GC
FspI 720 TGCGCA TGC^GCA
HhaI 721 GCGC GCG^C
HinP1I 721 GCGC G^CGC
HincII 308 GTCAAC GT[CT]^[GA]AC
HindII 308 GTCAAC GT[CT]^[GA]AC
HphI 1198 GGTGA GGTGA(8/7)
ItaI 55 GCAGC GC^[ACGT]GC
MaeII 621 ACGT A^CGT
MluNI 1221 TGGCCA TGG^CCA
MscI 1221 TGGCCA TGG^CCA
MseI 1358 TTAA T^TAA
MspA1I 56 CAGCTG C[AC]G^C[GT]G
MvaI 1164 CCAGG CC^[AT]GG
NcoI 1189 CCATGG C^CATGG
PvuII 56 CAGCTG CAG^CTG
SacI 775 GAGCTC GAGCT^C
SapI 595 GAAGAGC GCTCTTC(1/4)
Sau96I 132 GGGCC G^G[ACGT]CC
ScrFI 1164 CCAGG CC^[ACGT]GG
SfaNI 214 GATGC GCATC(5/9)
SmlI 428 CTCAAG C^T[CT][GA]AG
SspI 473 AATATT AAT^ATT
StyI 1189 CCATGG C^C[AT][AT]GG
Tru9I 1358 TTAA T^TAA
TseI 55 GCAGC G^C[AT]GC
TspRI 1332 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 135 CCAGCACTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 103 GAATTT [GA]^AATT[CT]
633 AAATTC
ApoI 103 GAATTT [GA]^AATT[CT]
633 AAATTC
AspHI 539 GTGCAC G[AT]GC[AT]^C
775 GAGCTC
BamHI 565 GGATCC G^GATCC
745 GGATCC
BciVI 925 GTATCC GTATCC(6/5)
1105 GTATCC
BmyI 539 GTGCAC G[AGT]GC[ACT]^C
775 GAGCTC
BsgI 913 GTGCAG GTGCAG(16/14)
1093 GTGCAG
BsiHKAI 539 GTGCAC G[AT]GC[AT]^C
775 GAGCTC
Bsp1286I 539 GTGCAC G[AGT]GC[ACT]^C
775 GAGCTC
BspHI 225 TCATGA T^CATGA
1257 TCATGA
EaeI 837 TGGCCG [CT]^GGCC[GA]
1221 TGGCCA
EarI 595 GAAGAG CTCTTC(1/4)
955 GAAGAG
Ksp632I 595 GAAGAG CTCTTC(1/4)
955 GAAGAG
PflMI 660 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1189 CCATGGCATGG
RcaI 225 TCATGA T^CATGA
1257 TCATGA
TfiI 172 GAATC G^A[AT]TC
795 GAATC
Van91I 660 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1189 CCATGGCATGG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
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AfaI AluI AlwI BfaI BsiYI
BslI BsmAI BsmFI BsrI BstYI
Cac8I DdeI DpnII HaeIII HinfI
MaeI MboI MboII MflI MnlI
MslI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Tsp509I XhoII