Restriction map for
pFN21AE4128
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaLI AseI AsnI Asp700I
Asp718I AspI BbvCI BciVI BclI
BfrI BsaBI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eco47III EcoO65I EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HinfI
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tth111I Van91I XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 301 GAATTC [GA]^AATT[CT]
AhdI 925 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1313 CCTCAGG CC^T[ACGT]AGG
ApaI 111 GGGCCC GGGCC^C
ApoI 301 GAATTC [GA]^AATT[CT]
AscI 1065 GGCGCGCC GG^CGCGCC
AspEI 925 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 125 GGATCC G^GATCC
BanII 111 GGGCCC G[GA]GC[CT]^C
BbrPI 370 CACGTG CAC^GTG
BbsI 391 GTCTTC GAAGAC(2/6)
BcgI 992 GCAAGGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 391 GTCTTC GAAGAC(2/6)
BsaAI 370 CACGTG [CT]AC^GT[GA]
BsaWI 41 TCCGGT [AT]^CCGG[AT]
BsmBI 672 CGTCTC CGTCTC(1/5)
BsrBI 910 CCGCTC CCGCTC(-3/-3)
Bsu36I 1313 CCTCAGG CC^T[ACGT]AGG
EagI 752 CGGCCG C^GGCCG
Eam1105I 925 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 661 CTCTTC CTCTTC(1/4)
EclXI 752 CGGCCG C^GGCCG
Eco52I 752 CGGCCG C^GGCCG
Eco81I 1313 CCTCAGG CC^T[ACGT]AGG
EcoNI 1008 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 301 GAATTC G^AATTC
HphI 1358 TCACC GGTGA(8/7)
Ksp632I 661 CTCTTC CTCTTC(1/4)
MaeIII 1288 GTGAC ^GT[ACGT]AC
MseI 1214 TTAA T^TAA
NcoI 626 CCATGG C^CATGG
NotI 751 GCGGCCGC GC^GGCCGC
PaeR7I 823 CTCGAG C^TCGAG
PmaCI 370 CACGTG CAC^GTG
PmlI 370 CACGTG CAC^GTG
SapI 660 GCTCTTC GCTCTTC(1/4)
SbfI 234 CCTGCAGG CCTGCA^GG
ScaI 827 AGTACT AGT^ACT
Sse8387I 234 CCTGCAGG CCTGCA^GG
Tru9I 1214 TTAA T^TAA
Tsp45I 1288 GTGAC ^GT[GC]AC
Tsp509I 302 AATT ^AATT
XbaI 1533 TCTAGA T^CTAGA
XcmI 1225 CCAGCCTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 823 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 126 GATCC GGATC(4/5)
125 GGATC
AvaII 15 GGTCC G^G[AT]CC
531 GGACC
AviII 1040 TGCGCA TGC^GCA
1421 TGCGCA
BalI 360 TGGCCA TGG^CCA
402 TGGCCA
BglII 548 AGATCT A^GATCT
1530 AGATCT
BlpI 186 GCTGAGC GC^T[ACGT]AGC
1455 GCTGAGC
Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC
1455 GCTGAGC
BsiEI 312 CGACCG CG[GA][CT]^CG
752 CGGCCG
BsmAI 1492 GAGAC GTCTC(1/5)
673 GTCTC
BsmFI 500 GGGAC GGGAC(10/14)
1518 GGGAC
BspMI 365 ACCTGC ACCTGC(4/8)
1023 GCAGGT
BsrFI 314 ACCGGC [GA]^CCGG[CT]
1391 ACCGGC
BstXI 677 CCATCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1225 CCAGCCTGCTGG
CelII 186 GCTGAGC GC^T[ACGT]AGC
1455 GCTGAGC
Cfr10I 314 ACCGGC [GA]^CCGG[CT]
1391 ACCGGC
FspI 1040 TGCGCA TGC^GCA
1421 TGCGCA
KspI 242 CCGCGG CCGC^GG
773 CCGCGG
MluNI 360 TGGCCA TGG^CCA
402 TGGCCA
MscI 360 TGGCCA TGG^CCA
402 TGGCCA
MslI 678 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1335 CATCCTGGTG
PstI 235 CTGCAG CTGCA^G
1526 CTGCAG
SacII 242 CCGCGG CCGC^GG
773 CCGCGG
SfcI 235 CTGCAG C^T[GA][CT]AG
1526 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvrII BanI BbeI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI MaeI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfoI
SmaI SmlI StyI TaqI TseI
TspRI TthHB8I XhoII XmaCI XmaI