Restriction map for
pFN21AE3004
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# NO CUT ENZYMES ( 204 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MboI MboII MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I TspRI Tth111I Van91I XbaI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 64 GCGG CCGC(-3/-1)
AhdI 48 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 89 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 48 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 139 CTCGAG C^[CT]CG[GA]G
BbvI 206 GCAGC GCAGC(8/12)
BpmI 116 CTGGAG CTGGAG(16/14)
BsaJI 78 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 44 ACCGGA [AT]^CCGG[AT]
BsmI 60 GAATGC GAATGC(1/-1)
BsoBI 139 CTCGAG C^[CT]CG[GA]G
BssKI 78 CCAGG ^CC[ACGT]GG
BstF5I 75 CATCC GGATG(2/0)
BstNI 78 CCAGG CC^[AT]GG
CfoI 170 GCGC GCG^C
DdeI 87 CTCAG C^T[ACGT]AG
DrdI 216 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 48 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 78 CCAGG ^CC[AT]GG
Fnu4HI 206 GCAGC GC^[ACGT]GC
FokI 75 CATCC GGATG(9/13)
HaeII 169 AGCGCC [GA]GCGC^[CT]
HaeIII 52 GGCC GG^CC
HapII 45 CCGG C^CGG
HhaI 170 GCGC GCG^C
HinP1I 170 GCGC G^CGC
HpaII 45 CCGG C^CGG
ItaI 206 GCAGC GC^[ACGT]GC
MaeIII 41 GTGAC ^GT[ACGT]AC
MspI 45 CCGG C^CGG
MvaI 78 CCAGG CC^[AT]GG
NlaIII 123 CATG CATG^
PaeR7I 139 CTCGAG C^TCGAG
ScrFI 78 CCAGG CC^[ACGT]GG
SmlI 139 CTCGAG C^T[CT][GA]AG
TseI 206 GCAGC G^C[AT]GC
Tsp45I 41 GTGAC ^GT[GC]AC
XcmI 201 CCAGAGCAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 139 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 66 GGTCC G^G[AT]CC
215 GGACC
BsiYI 38 CCTGTGACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
174 CCCAGACAAGG
BslI 38 CCTGTGACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
174 CCCAGACAAGG
BsmAI 83 GAGAC GTCTC(1/5)
119 GAGAC
BsmFI 214 GGGAC GGGAC(10/14)
67 GTCCC
Cfr13I 66 GGTCC G^G[ACGT]CC
215 GGACC
HinfI 13 GAGTC G^A[ACGT]TC
85 GACTC
NlaIV 66 GGTCCC GG[ACGT]^[ACGT]CC
214 GGGACC
PleI 85 GACTC GAGTC(4/5)
13 GAGTC
Sau96I 66 GGTCC G^G[ACGT]CC
215 GGACC
TaqI 11 TCGA T^CGA
140 TCGA
TthHB8I 11 TCGA T^CGA
140 TCGA
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
AluI Cac8I MnlI