##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tsp45I      Tsp509I     TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AfaI           39  GTAC                 GT^AC
AluI          295  AGCT                 AG^CT
Alw44I         84  GTGCAC               G^TGCAC
ApaLI          84  GTGCAC               G^TGCAC
BanII         294  GAGCTC               G[GA]GC[CT]^C
BbvI          302  GCAGC                GCAGC(8/12)
BcgI          360  CGAGACCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         184  GGATAC               GTATCC(6/5)
BglI           92  GCCCCAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          189  CTGGAG               CTGGAG(16/14)
BsmFI         273  GGGAC                GGGAC(10/14)
BspMI         145  GCAGGT               ACCTGC(4/8)
BsrI          188  ACTGG                ACTGG(1/-1)
BstF5I        380  GGATG                GGATG(2/0)
BstXI          69  CCATGGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         236  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          234  GCGC                 GCG^C
DraII          90  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI           69  CCATGG               C^C[GA][CT]GG
EcoO109I       90  AGGCCCC              [GA]G^G[ACGT]CC[CT]
FauI           44  GCGGG                CCCGC(4/6)
FokI          380  GGATG                GGATG(9/13)
HaeII         233  GGCGCT               [GA]GCGC^[CT]
HapII         288  CCGG                 C^CGG
HgaI          275  GACGC                GACGC(5/10)
HhaI          234  GCGC                 GCG^C
HinP1I        234  GCGC                 G^CGC
HinfI         284  GAATC                G^A[ACGT]TC
HpaII         288  CCGG                 C^CGG
MslI           70  CATGGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          288  CCGG                 C^CGG
NcoI           69  CCATGG               C^CATGG
RsaI           39  GTAC                 GT^AC
SacI          294  GAGCTC               GAGCT^C
SfiI           91  GGCCCCAAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          297  CTCAAG               C^T[CT][GA]AG
TaqI           17  TCGA                 T^CGA
TfiI          284  GAATC                G^A[AT]TC
TseI          302  GCAGC                G^C[AT]GC
TthHB8I        17  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AspHI          84  GTGCAC               G[AT]GC[AT]^C
              294  GAGCTC              
BsaI          223  GAGACC               GGTCTC(1/5)
              361  GAGACC              
BsiHKAI        84  GTGCAC               G[AT]GC[AT]^C
              294  GAGCTC              
BsmAI         223  GAGAC                GTCTC(1/5)
              361  GAGAC               
Fnu4HI         11  GCCGC                GC^[ACGT]GC
              302  GCAGC               
ItaI           11  GCCGC                GC^[ACGT]GC
              302  GCAGC               
MspA1I        165  CAGCGG               C[AC]G^C[GT]G
              303  CAGCGG              
MwoI           92  GCCCCAAGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              296  GCTCAAGCAGC         


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AciI        BanI        BmyI        BsaJI       Bsp1286I    
BssKI       BstNI       Cfr13I      EcoRII      EcoT14I     
HaeIII      MnlI        MvaI        NlaIII      NlaIV       
Sau96I      ScrFI       StyI