Restriction map for
pFN21AE4162
##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AflII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspI AviII AvrII BamHI
BbeI BbrPI BfrI BglI BglII
BlnI BsaAI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FspI HaeII HindIII HpaI
KasI MflI MluI MroI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XhoII
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 1159 GTAGAC GT^[AC][GT]AC
AclI 1468 AACGTT AA^CGTT
AcsI 1343 GAATTC [GA]^AATT[CT]
AcyI 28 GACGCC G[GA]^CG[CT]C
AflIII 11 ACGTGT A^C[GA][CT]GT
AhdI 106 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 228 GGGCCC GGGCC^C
ApoI 1343 GAATTC [GA]^AATT[CT]
AspEI 106 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1008 TGGCCA TGG^CCA
BbsI 1554 GTCTTC GAAGAC(2/6)
BclI 384 TGATCA T^GATCA
BlpI 1111 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1111 GCTAAGC GC^T[ACGT]AGC
BpuAI 1554 GTCTTC GAAGAC(2/6)
BsaBI 1231 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 28 GACGCC G[GA]^CG[CT]C
BsmBI 670 CGTCTC CGTCTC(1/5)
BspMI 234 GCAGGT ACCTGC(4/8)
BsrBI 1752 CCGCTC CCGCTC(-3/-3)
BssSI 1610 CTCGTG CACGAG(-5/-1)
BstAPI 897 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1111 GCTAAGC GC^T[ACGT]AGC
DsaI 198 CCGCGG C^C[GA][CT]GG
EaeI 1008 TGGCCA [CT]^GGCC[GA]
Eam1105I 106 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1343 GAATTC G^AATTC
EcoT14I 476 CCAAGG C^C[AT][AT]GG
FbaI 384 TGATCA T^GATCA
FseI 60 GGCCGGCC GGCCGG^CC
Hin1I 28 GACGCC G[GA]^CG[CT]C
KspI 198 CCGCGG CCGC^GG
MamI 1231 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 901 CAATTG C^AATTG
MluNI 1008 TGGCCA TGG^CCA
MscI 1008 TGGCCA TGG^CCA
MseI 1301 TTAA T^TAA
MunI 901 CAATTG C^AATTG
NaeI 61 GCCGGC GCC^GGC
NgoMIV 61 GCCGGC G^CCGGC
NspI 579 GCATGT [GA]CATG^[CT]
PaeR7I 1144 CTCGAG C^TCGAG
PflMI 351 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 154 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1468 AACGTT AA^CGTT
SacII 198 CCGCGG CCGC^GG
SapI 1063 GAAGAGC GCTCTTC(1/4)
SmaI 145 CCCGGG CCC^GGG
StuI 1745 AGGCCT AGG^CCT
StyI 476 CCAAGG C^C[AT][AT]GG
Tru9I 1301 TTAA T^TAA
Van91I 351 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 999 TCTAGA T^CTAGA
XhoI 1144 CTCGAG C^TCGAG
XmaCI 145 CCCGGG C^CCGGG
XmaI 145 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 90 GGTACC G^GTACC
1433 GGTACC
AlwNI 352 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
404 CAGAAGCTG
Asp718I 90 GGTACC G^GTACC
1433 GGTACC
BbvCI 714 CCTCAGC CCTCAGC(-5/-2)
757 GCTGAGG
BcgI 27 CGACGCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1580 CGAACCCTGTGC
BciVI 565 GGATAC GTATCC(6/5)
1497 GTATCC
BfaI 1000 CTAG C^TAG
1633 CTAG
BsaI 1174 GAGACC GGTCTC(1/5)
1542 GAGACC
BsaWI 42 ACCGGA [AT]^CCGG[AT]
1550 TCCGGT
BsgI 540 GTGCAG GTGCAG(16/14)
988 GTGCAG
BsiEI 201 CGGTCG CG[GA][CT]^CG
294 CGACCG
BstEII 658 GGTTACC G^GT[ACGT]ACC
1278 GGTCACC
BstPI 658 GGTTACC G^GT[ACGT]ACC
1278 GGTCACC
BstXI 265 CCACTGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
648 CCAAGCAGCTGG
DraII 152 AGGACCC [GA]G^G[ACGT]CC[CT]
159 AGGTCCC
EcoO109I 152 AGGACCC [GA]G^G[ACGT]CC[CT]
159 AGGTCCC
EcoO65I 658 GGTTACC G^GT[ACGT]ACC
1278 GGTCACC
HincII 310 GTCAAC GT[CT]^[GA]AC
1138 GTCAAC
HindII 310 GTCAAC GT[CT]^[GA]AC
1138 GTCAAC
KpnI 90 GGTACC GGTAC^C
1433 GGTACC
MaeI 1000 CTAG C^TAG
1633 CTAG
MslI 355 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1630 CACCTAGATG
PleI 1054 GACTC GAGTC(4/5)
1410 GACTC
PpuMI 152 AGGACCC [GA]G^G[AT]CC[CT]
159 AGGTCCC
SacI 919 GAGCTC GAGCT^C
1349 GAGCTC
SmlI 1144 CTCGAG C^T[CT][GA]AG
1693 CTCAAG
TfiI 471 GATTC G^A[AT]TC
732 GATTC
Tsp45I 1279 GTCAC ^GT[GC]AC
1613 GTGAC
XcmI 120 CCAAGTCCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
842 CCAGCAAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
Asp700I AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp509I TspRI TthHB8I XmnI