Restriction map for
pFN21AE5087
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstNI BstPI BstUI BstYI
BstZ17I Bsu36I Cac8I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI KspI MaeI MaeII MaeIII
MfeI MflI MluI MnlI MroI
MseI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AlwI 131 GGATC GGATC(4/5)
AspI 49 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 16 GGTCC G^G[AT]CC
BalI 212 TGGCCA TGG^CCA
BbvI 41 GCTGC GCAGC(8/12)
BcnI 98 CCGGG CC^[GC]GG
BsaBI 222 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 122 CCTGTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 122 CCTGTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 125 GTCTC GTCTC(1/5)
BsmI 137 GAATGC GAATGC(1/-1)
BsrFI 3 GCCGGT [GA]^CCGG[CT]
BssKI 98 CCGGG ^CC[ACGT]GG
BstAPI 227 GCATCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 215 CCATTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3 GCCGGT [GA]^CCGG[CT]
Cfr13I 16 GGTCC G^G[ACGT]CC
DdeI 127 CTCAG C^T[ACGT]AG
DpnII 132 GATC ^GATC
DraII 15 GGGTCCC [GA]G^G[ACGT]CC[CT]
EagI 95 CGGCCG C^GGCCG
EarI 106 CTCTTC CTCTTC(1/4)
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoNI 122 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 15 GGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 141 GCGGG CCCGC(4/6)
HaeII 102 GGCGCT [GA]GCGC^[CT]
Ksp632I 106 CTCTTC CTCTTC(1/4)
MamI 222 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 132 GATC ^GATC
MboII 107 TCTTC GAAGA(8/7)
MluNI 212 TGGCCA TGG^CCA
MscI 212 TGGCCA TGG^CCA
NciI 98 CCGGG CC^[GC]GG
NdeII 132 GATC ^GATC
PflFI 49 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 15 GGGTCCC [GA]G^G[AT]CC[CT]
SapI 105 GCTCTTC GCTCTTC(1/4)
Sau3AI 132 GATC ^GATC
Sau96I 16 GGTCC G^G[ACGT]CC
ScrFI 98 CCGGG CC^[ACGT]GG
TseI 41 GCTGC G^C[AT]GC
Tth111I 49 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 40 AGCT AG^CT
152 AGCT
BanI 112 GGCACC G^G[CT][GA]CC
193 GGCACC
BsiEI 48 CGACCG CG[GA][CT]^CG
95 CGGCCG
BsmFI 17 GTCCC GGGAC(10/14)
30 GTCCC
BstF5I 228 CATCC GGATG(2/0)
221 GGATG
CfoI 80 GCGC GCG^C
103 GCGC
DsaI 11 CCGTGG C^C[GA][CT]GG
83 CCGTGG
FokI 228 CATCC GGATG(9/13)
221 GGATG
HapII 4 CCGG C^CGG
98 CCGG
HhaI 80 GCGC GCG^C
103 GCGC
HinP1I 80 GCGC G^CGC
103 GCGC
HpaII 4 CCGG C^CGG
98 CCGG
MslI 204 CACATTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
216 CATTGGGATG
MspI 4 CCGG C^CGG
98 CCGG
TaqI 47 TCGA T^CGA
147 TCGA
TthHB8I 47 TCGA T^CGA
147 TCGA
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
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AciI BsaJI EaeI Fnu4HI HaeIII
ItaI MwoI NlaIII NlaIV SfaNI