Restriction map for
pFN21AE1946
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspHI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII Hin1I HindIII
HpaI KasI KspI MaeI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 35 GGTACC G^GTACC
AcsI 58 GAATTC [GA]^AATT[CT]
AflII 1186 CTTAAG C^TTAAG
AflIII 665 ACGTGT A^C[GA][CT]GT
AhdI 1369 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 58 GAATTC [GA]^AATT[CT]
Asp718I 35 GGTACC G^GTACC
AspEI 1369 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 169 GTCTTC GAAGAC(2/6)
BfrI 1186 CTTAAG C^TTAAG
BpuAI 169 GTCTTC GAAGAC(2/6)
BsaAI 664 TACGTG [CT]AC^GT[GA]
BsaI 99 GAGACC GGTCTC(1/5)
BseRI 16 CTCCTC GAGGAG(10/8)
BsgI 66 CTGCAC GTGCAG(16/14)
Bsp1407I 527 TGTACA T^GTACA
BsrBI 1080 CCGCTC CCGCTC(-3/-3)
BsrDI 1307 CATTGC GCAATG(2/0)
BsrGI 527 TGTACA T^GTACA
BstXI 1445 CCAACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 1411 CTGAG C^T[ACGT]AG
EagI 810 CGGCCG C^GGCCG
Eam1105I 1369 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 810 CGGCCG C^GGCCG
Eco52I 810 CGGCCG C^GGCCG
EcoNI 684 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 58 GAATTC G^AATTC
HgaI 534 GACGC GACGC(5/10)
HincII 1219 GTCAAC GT[CT]^[GA]AC
HindII 1219 GTCAAC GT[CT]^[GA]AC
HphI 605 TCACC GGTGA(8/7)
KpnI 35 GGTACC GGTAC^C
NcoI 1364 CCATGG C^CATGG
PshAI 634 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 894 GCTCTTC GCTCTTC(1/4)
SfiI 1143 GGCCCCACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 454 CCCGGG CCC^GGG
SspBI 527 TGTACA T^GTACA
TfiI 1332 GAATC G^A[AT]TC
Tsp509I 59 AATT ^AATT
XcmI 113 CCAGGCCAGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 454 CCCGGG C^CCGGG
XmaI 454 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 991 CGCG CG^CG
1406 CGCG
AlwNI 410 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1348 CAGACCCTG
ApaI 457 GGGCCC GGGCC^C
1142 GGGCCC
Asp700I 165 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
891 GAAGCTCTTC
AspI 534 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACATGGTC
AvaI 454 CCCGGG C^[CT]CG[GA]G
1372 CCCGAG
BsiEI 810 CGGCCG CG[GA][CT]^CG
987 CGACCG
BsmAI 3 GAGAC GTCTC(1/5)
99 GAGAC
BsoBI 454 CCCGGG C^[CT]CG[GA]G
1372 CCCGAG
BspMI 1390 ACCTGC ACCTGC(4/8)
1002 GCAGGT
BsrFI 812 GCCGGC [GA]^CCGG[CT]
819 GCCGGC
BsrI 1149 ACTGG ACTGG(1/-1)
1247 ACTGG
BstUI 991 CGCG CG^CG
1406 CGCG
Cfr10I 812 GCCGGC [GA]^CCGG[CT]
819 GCCGGC
EarI 895 CTCTTC CTCTTC(1/4)
1429 CTCTTC
Ksp632I 895 CTCTTC CTCTTC(1/4)
1429 CTCTTC
MaeIII 185 GTCAC ^GT[ACGT]AC
364 GTGAC
MslI 187 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1476 CATGGTCGTG
MvnI 991 CGCG CG^CG
1406 CGCG
NaeI 812 GCCGGC GCC^GGC
819 GCCGGC
NgoMIV 812 GCCGGC G^CCGGC
819 GCCGGC
NspI 515 ACATGC [GA]CATG^[CT]
1215 GCATGT
PflFI 534 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACATGGTC
PpuMI 639 AGGTCCC [GA]G^G[AT]CC[CT]
1052 GGGACCT
PstI 1197 CTGCAG CTGCA^G
1497 CTGCAG
SfaNI 544 GATGC GCATC(5/9)
1424 GCATC
SmlI 1186 CTTAAG C^T[CT][GA]AG
1264 CTTGAG
TaqI 881 TCGA T^CGA
986 TCGA
Tsp45I 185 GTCAC ^GT[GC]AC
364 GTGAC
Tth111I 534 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1474 GACATGGTC
TthHB8I 881 TCGA T^CGA
986 TCGA
XmnI 165 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
891 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BssSI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StuI StyI Tru9I TseI
TspRI XhoII