Restriction map for
pFN21AE6551
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# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BpmI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 376 CGCG CG^CG
AlwNI 360 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 76 CCTGAGG CC^T[ACGT]AGG
AspHI 379 GTGCTC G[AT]GC[AT]^C
AvaII 257 GGACC G^G[AT]CC
BanI 309 GGCACC G^G[CT][GA]CC
BbsI 37 GTCTTC GAAGAC(2/6)
BcnI 254 CCCGG CC^[GC]GG
BglII 211 AGATCT A^GATCT
BlpI 381 GCTCAGC GC^T[ACGT]AGC
BmyI 379 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 381 GCTCAGC GC^T[ACGT]AGC
BpuAI 37 GTCTTC GAAGAC(2/6)
BsaJI 253 CCCCGG C^C[ACGT][ACGT]GG
BsiHKAI 379 GTGCTC G[AT]GC[AT]^C
BsmAI 148 GTCTC GTCTC(1/5)
BsmFI 139 GTCCC GGGAC(10/14)
Bsp1286I 379 GTGCTC G[AGT]GC[ACT]^C
BspMI 68 ACCTGC ACCTGC(4/8)
BsrFI 306 GCCGGC [GA]^CCGG[CT]
BstNI 21 CCTGG CC^[AT]GG
BstUI 376 CGCG CG^CG
BstXI 26 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 211 AGATCT [GA]^GATC[CT]
Bsu36I 76 CCTGAGG CC^T[ACGT]AGG
CelII 381 GCTCAGC GC^T[ACGT]AGC
Cfr10I 306 GCCGGC [GA]^CCGG[CT]
Eco81I 76 CCTGAGG CC^T[ACGT]AGG
EcoRII 21 CCTGG ^CC[AT]GG
HphI 8 TCACC GGTGA(8/7)
MaeIII 7 GTCAC ^GT[ACGT]AC
MflI 211 AGATCT [GA]^GATC[CT]
MspA1I 294 CAGCTG C[AC]G^C[GT]G
MvaI 21 CCTGG CC^[AT]GG
MvnI 376 CGCG CG^CG
NaeI 306 GCCGGC GCC^GGC
NciI 254 CCCGG CC^[GC]GG
NgoMIV 306 GCCGGC G^CCGGC
PvuII 294 CAGCTG CAG^CTG
TaqI 232 TCGA T^CGA
Tsp45I 7 GTCAC ^GT[GC]AC
TthHB8I 232 TCGA T^CGA
XhoII 211 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 86 AGCT AG^CT
295 AGCT
BalI 23 TGGCCA TGG^CCA
281 TGGCCA
BseRI 117 CTCCTC GAGGAG(10/8)
79 GAGGAG
BsrI 132 ACTGG ACTGG(1/-1)
342 CCAGT
BssKI 21 CCTGG ^CC[ACGT]GG
254 CCCGG
BstAPI 290 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
359 GCAGCGCCTGC
BstF5I 3 GGATG GGATG(2/0)
135 GGATG
Cfr13I 251 GGCCC G^G[ACGT]CC
257 GGACC
DpnII 183 GATC ^GATC
212 GATC
EaeI 23 TGGCCA [CT]^GGCC[GA]
281 TGGCCA
FokI 3 GGATG GGATG(9/13)
135 GGATG
HaeII 159 GGCGCT [GA]GCGC^[CT]
361 AGCGCC
HapII 255 CCGG C^CGG
307 CCGG
HpaII 255 CCGG C^CGG
307 CCGG
MboI 183 GATC ^GATC
212 GATC
MluNI 23 TGGCCA TGG^CCA
281 TGGCCA
MscI 23 TGGCCA TGG^CCA
281 TGGCCA
MspI 255 CCGG C^CGG
307 CCGG
NdeII 183 GATC ^GATC
212 GATC
Sau3AI 183 GATC ^GATC
212 GATC
Sau96I 251 GGCCC G^G[ACGT]CC
257 GGACC
ScrFI 21 CCTGG CC^[ACGT]GG
254 CCCGG
TspRI 54 CACTG CA[GC]TG[ACGT][ACGT]^
343 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AciI BbvI Cac8I CfoI DdeI
FauI Fnu4HI HaeIII HhaI HinP1I
ItaI MboII MnlI MwoI NlaIV
TseI