Restriction map for
pFN21AE5254
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BclI BfaI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI Hin1I HindIII HinfI
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 384 GTAC GT^AC
AflII 88 CTTAAG C^TTAAG
AlwI 524 GATCC GGATC(4/5)
ApaI 131 GGGCCC GGGCC^C
AvaI 328 CTCGGG C^[CT]CG[GA]G
AvaII 459 GGACC G^G[AT]CC
AviII 53 TGCGCA TGC^GCA
BciVI 335 GTATCC GTATCC(6/5)
BcnI 129 CCGGG CC^[GC]GG
BfrI 88 CTTAAG C^TTAAG
BpmI 178 CTCCAG CTGGAG(16/14)
BsaI 259 GGTCTC GGTCTC(1/5)
BsmAI 260 GTCTC GTCTC(1/5)
BsmI 310 GCATTC GAATGC(1/-1)
BsoBI 328 CTCGGG C^[CT]CG[GA]G
BspHI 149 TCATGA T^CATGA
BspLU11I 380 ACATGT A^CATGT
BsrDI 578 GCAATG GCAATG(2/0)
BstF5I 441 CATCC GGATG(2/0)
EaeI 80 TGGCCG [CT]^GGCC[GA]
EarI 160 CTCTTC CTCTTC(1/4)
EcoNI 564 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 441 CATCC GGATG(9/13)
FspI 53 TGCGCA TGC^GCA
HaeII 59 AGCGCC [GA]GCGC^[CT]
HgaI 398 GCGTC GACGC(5/10)
HincII 40 GTCAAC GT[CT]^[GA]AC
HindII 40 GTCAAC GT[CT]^[GA]AC
Ksp632I 160 CTCTTC CTCTTC(1/4)
MaeIII 393 GTCAC ^GT[ACGT]AC
MboII 161 TCTTC GAAGA(8/7)
MluI 396 ACGCGT A^CGCGT
MseI 89 TTAA T^TAA
MslI 477 CACTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 469 CCGCTG C[AC]G^C[GT]G
NciI 129 CCGGG CC^[GC]GG
NspI 380 ACATGT [GA]CATG^[CT]
PpuMI 458 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 149 TCATGA T^CATGA
RsaI 384 GTAC GT^AC
SmlI 88 CTTAAG C^T[CT][GA]AG
Tru9I 89 TTAA T^TAA
Tsp45I 393 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 141 CGCG CG^CG
397 CGCG
AccIII 98 TCCGGA T^CCGGA
164 TCCGGA
AflIII 380 ACATGT A^C[GA][CT]GT
396 ACGCGT
AluI 17 AGCT AG^CT
188 AGCT
BanII 23 GGGCTC G[GA]GC[CT]^C
131 GGGCCC
BsaJI 327 CCTCGG C^C[ACGT][ACGT]GG
451 CCCAGG
BsaWI 98 TCCGGA [AT]^CCGG[AT]
164 TCCGGA
BseAI 98 TCCGGA T^CCGGA
164 TCCGGA
BsmFI 458 GGGAC GGGAC(10/14)
626 GTCCC
BspEI 98 TCCGGA T^CCGGA
164 TCCGGA
BstUI 141 CGCG CG^CG
397 CGCG
Cac8I 56 GCAAGC GC[ACGT]^[ACGT]GC
204 GCAGGC
DpnII 375 GATC ^GATC
524 GATC
DraII 131 GGGCCCC [GA]G^G[ACGT]CC[CT]
458 GGGACCC
EcoO109I 131 GGGCCCC [GA]G^G[ACGT]CC[CT]
458 GGGACCC
FauI 9 CCCGC CCCGC(4/6)
468 CCCGC
HphI 248 TCACC GGTGA(8/7)
519 TCACC
MboI 375 GATC ^GATC
524 GATC
MroI 98 TCCGGA T^CCGGA
164 TCCGGA
MvnI 141 CGCG CG^CG
397 CGCG
NdeII 375 GATC ^GATC
524 GATC
Sau3AI 375 GATC ^GATC
524 GATC
TspRI 47 CACTG CA[GC]TG[ACGT][ACGT]^
477 CACTG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI BbvI BmyI BsiYI BslI
Bsp1286I BssKI BstNI CfoI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspI MvaI MwoI NlaIII NlaIV
PstI Sau96I ScrFI SfcI TseI