Restriction map for
pFN21AE5278
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 180 GGTACC G^GTACC
Alw44I 256 GTGCAC G^TGCAC
AlwI 69 GGATC GGATC(4/5)
AocI 175 CCTGAGG CC^T[ACGT]AGG
ApaLI 256 GTGCAC G^TGCAC
Asp718I 180 GGTACC G^GTACC
AspHI 256 GTGCAC G[AT]GC[AT]^C
AvaI 614 CCCGGG C^[CT]CG[GA]G
BcgI 155 GCATCCCGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 325 CTAG C^TAG
BpmI 448 CTGGAG CTGGAG(16/14)
BsiHKAI 256 GTGCAC G[AT]GC[AT]^C
BsoBI 614 CCCGGG C^[CT]CG[GA]G
BsrDI 5 GCAATG GCAATG(2/0)
BsrI 422 CCAGT ACTGG(1/-1)
BstAPI 515 GCAAGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 59 CCAAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 175 CCTGAGG CC^T[ACGT]AGG
EaeI 628 CGGCCG [CT]^GGCC[GA]
EagI 628 CGGCCG C^GGCCG
EarI 406 GAAGAG CTCTTC(1/4)
EclXI 628 CGGCCG C^GGCCG
Eco52I 628 CGGCCG C^GGCCG
Eco81I 175 CCTGAGG CC^T[ACGT]AGG
EcoRV 139 GATATC GAT^ATC
EcoT14I 17 CCAAGG C^C[AT][AT]GG
HgaI 650 GCGTC GACGC(5/10)
HindIII 433 AAGCTT A^AGCTT
KpnI 180 GGTACC GGTAC^C
Ksp632I 406 GAAGAG CTCTTC(1/4)
MaeI 325 CTAG C^TAG
MaeII 338 ACGT A^CGT
MboII 406 GAAGA GAAGA(8/7)
MseI 236 TTAA T^TAA
MslI 288 CACGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
SmaI 614 CCCGGG CCC^GGG
StyI 17 CCAAGG C^C[AT][AT]GG
Tru9I 236 TTAA T^TAA
XmaCI 614 CCCGGG C^CCGGG
XmaI 614 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 22 GTAC GT^AC
181 GTAC
AlwNI 124 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
592 CAGCGCCTG
Aor51HI 455 AGCGCT AGC^GCT
518 AGCGCT
BanI 180 GGTACC G^G[CT][GA]CC
600 GGTGCC
BanII 468 GAGCCC G[GA]GC[CT]^C
531 GGGCTC
BglI 349 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
524 GCCGGCAGGGC
BsiEI 161 CGGTCG CG[GA][CT]^CG
628 CGGCCG
BsmFI 335 GGGAC GGGAC(10/14)
687 GTCCC
BstF5I 156 CATCC GGATG(2/0)
219 CATCC
DdeI 176 CTGAG C^T[ACGT]AG
567 CTCAG
DpnII 70 GATC ^GATC
90 GATC
Eco47III 455 AGCGCT AGC^GCT
518 AGCGCT
FauI 689 CCCGC CCCGC(4/6)
333 GCGGG
FokI 156 CATCC GGATG(9/13)
219 CATCC
HinfI 32 GACTC G^A[ACGT]TC
115 GAGTC
HphI 118 TCACC GGTGA(8/7)
143 TCACC
MboI 70 GATC ^GATC
90 GATC
NaeI 440 GCCGGC GCC^GGC
524 GCCGGC
NdeII 70 GATC ^GATC
90 GATC
NgoMIV 440 GCCGGC G^CCGGC
524 GCCGGC
NlaIII 12 CATG CATG^
112 CATG
PleI 32 GACTC GAGTC(4/5)
115 GAGTC
PstI 133 CTGCAG CTGCA^G
589 CTGCAG
PvuII 73 CAGCTG CAG^CTG
445 CAGCTG
RsaI 22 GTAC GT^AC
181 GTAC
Sau3AI 70 GATC ^GATC
90 GATC
SfcI 133 CTGCAG C^T[GA][CT]AG
589 CTGCAG
TspRI 223 CACTG CA[GC]TG[ACGT][ACGT]^
259 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
Bsp1286I BsrFI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
EcoRII Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
SfaNI TseI Tsp45I