Restriction map for
pFN21AE1651
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraIII DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MamI
MboI MboII MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AluI 367 AGCT AG^CT
AspHI 45 GAGCAC G[AT]GC[AT]^C
AvaI 268 CTCGAG C^[CT]CG[GA]G
AvaII 84 GGACC G^G[AT]CC
BanI 414 GGTGCC G^G[CT][GA]CC
BbrPI 95 CACGTG CAC^GTG
BfaI 235 CTAG C^TAG
BpmI 400 CTCCAG CTGGAG(16/14)
BsaAI 95 CACGTG [CT]AC^GT[GA]
BsgI 186 CTGCAC GTGCAG(16/14)
BsiHKAI 45 GAGCAC G[AT]GC[AT]^C
BsiYI 312 CCTGCGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 312 CCTGCGTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 216 GTCCC GGGAC(10/14)
BsoBI 268 CTCGAG C^[CT]CG[GA]G
BspLU11I 197 ACATGT A^CATGT
BsrBI 5 GAGCGG CCGCTC(-3/-3)
BsrI 52 ACTGG ACTGG(1/-1)
BstF5I 321 GGATG GGATG(2/0)
DdeI 364 CTGAG C^T[ACGT]AG
DrdI 136 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 218 CCCGC CCCGC(4/6)
FokI 321 GGATG GGATG(9/13)
HgaI 315 GCGTC GACGC(5/10)
MaeI 235 CTAG C^TAG
MaeII 96 ACGT A^CGT
MseI 89 TTAA T^TAA
NlaIII 198 CATG CATG^
NspI 197 ACATGT [GA]CATG^[CT]
PaeR7I 268 CTCGAG C^TCGAG
PmaCI 95 CACGTG CAC^GTG
PmlI 95 CACGTG CAC^GTG
PpuMI 83 AGGACCT [GA]G^G[AT]CC[CT]
SfaNI 221 GCATC GCATC(5/9)
StuI 252 AGGCCT AGG^CCT
Tru9I 89 TTAA T^TAA
Tsp509I 279 AATT ^AATT
XhoI 268 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AciI 219 CCGC CCGC(-3/-1)
7 GCGG
AflIII 96 ACGTGT A^C[GA][CT]GT
197 ACATGT
AlwNI 307 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
358 CAGACTCTG
ApaI 57 GGGCCC GGGCC^C
230 GGGCCC
BanII 57 GGGCCC G[GA]GC[CT]^C
230 GGGCCC
BspMI 292 ACCTGC ACCTGC(4/8)
311 ACCTGC
Cac8I 3 GCGAGC GC[ACGT]^[ACGT]GC
21 GCTGGC
MaeIII 329 GTGAC ^GT[ACGT]AC
431 GTCAC
SmlI 42 CTTGAG C^T[CT][GA]AG
268 CTCGAG
TaqI 269 TCGA T^CGA
410 TCGA
Tsp45I 329 GTGAC ^GT[GC]AC
431 GTCAC
TspRI 51 CACTG CA[GC]TG[ACGT][ACGT]^
165 CAGTG
TthHB8I 269 TCGA T^CGA
410 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
BbvI BmyI BsaJI Bsp1286I BssKI
BstNI Cfr13I DraII EcoO109I EcoRII
Fnu4HI HaeIII HinfI ItaI MnlI
MvaI NlaIV PleI Sau96I ScrFI
TseI