Restriction map for
pFN21AE2017
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# NO CUT ENZYMES ( 113 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaHI BsiWI BsmI
BsoBI Bsp1407I BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1608 GTCTAC GT^[AC][GT]AC
AccIII 591 TCCGGA T^CCGGA
Alw44I 914 GTGCAC G^TGCAC
ApaLI 914 GTGCAC G^TGCAC
Asp700I 1752 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 953 TGGCCA TGG^CCA
BamHI 288 GGATCC G^GATCC
BanII 1010 GAGCCC G[GA]GC[CT]^C
BbrPI 13 CACGTG CAC^GTG
BbvCI 355 GCTGAGG CCTCAGC(-5/-2)
BcgI 1141 CGAATCGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 960 GTATCC GTATCC(6/5)
BclI 500 TGATCA T^GATCA
BcnI 486 CCGGG CC^[GC]GG
BfaI 1307 CTAG C^TAG
BsaAI 13 CACGTG [CT]AC^GT[GA]
BsaBI 783 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 591 TCCGGA [AT]^CCGG[AT]
BseAI 591 TCCGGA T^CCGGA
BsiEI 32 CGGTCG CG[GA][CT]^CG
BspDI 1144 ATCGAT AT^CGAT
BspEI 591 TCCGGA T^CCGGA
BsrDI 1270 GCAATG GCAATG(2/0)
BsrFI 1698 GCCGGT [GA]^CCGG[CT]
BssSI 1167 CACGAG CACGAG(-5/-1)
Cfr10I 1698 GCCGGT [GA]^CCGG[CT]
ClaI 1144 ATCGAT AT^CGAT
DrdI 408 GACCGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 752 CCATGG C^C[GA][CT]GG
FbaI 500 TGATCA T^GATCA
HaeII 51 GGCGCT [GA]GCGC^[CT]
HgaI 1378 GCGTC GACGC(5/10)
HindIII 1186 AAGCTT A^AGCTT
MaeI 1307 CTAG C^TAG
MaeIII 1028 GTAAC ^GT[ACGT]AC
MamI 783 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 953 TGGCCA TGG^CCA
MroI 591 TCCGGA T^CCGGA
MscI 953 TGGCCA TGG^CCA
NciI 486 CCGGG CC^[GC]GG
NcoI 752 CCATGG C^CATGG
NspI 930 ACATGC [GA]CATG^[CT]
PmaCI 13 CACGTG CAC^GTG
PmlI 13 CACGTG CAC^GTG
PstI 82 CTGCAG CTGCA^G
SapI 1204 GAAGAGC GCTCTTC(1/4)
SfcI 82 CTGCAG C^T[GA][CT]AG
XcmI 512 CCATTAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1752 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 121 AAATTC [GA]^AATT[CT]
1681 AAATTC
AocI 1471 CCTCAGG CC^T[ACGT]AGG
1639 CCTGAGG
ApoI 121 AAATTC [GA]^AATT[CT]
1681 AAATTC
BanI 301 GGTGCC G^G[CT][GA]CC
1161 GGTGCC
BsaI 241 GAGACC GGTCTC(1/5)
1570 GAGACC
BsmBI 919 CGTCTC CGTCTC(1/5)
1420 GAGACG
BsmFI 444 GGGAC GGGAC(10/14)
155 GTCCC
BspHI 216 TCATGA T^CATGA
1259 TCATGA
BstYI 288 GGATCC [GA]^GATC[CT]
980 AGATCC
Bsu36I 1471 CCTCAGG CC^T[ACGT]AGG
1639 CCTGAGG
EaeI 953 TGGCCA [CT]^GGCC[GA]
1071 TGGCCG
Eco81I 1471 CCTCAGG CC^T[ACGT]AGG
1639 CCTGAGG
MaeII 14 ACGT A^CGT
918 ACGT
MflI 288 GGATCC [GA]^GATC[CT]
980 AGATCC
MslI 753 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1654 CACAGAAGTG
PflMI 387 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1036 CCAAAATATGG
PpuMI 221 AGGACCT [GA]G^G[AT]CC[CT]
359 AGGTCCT
RcaI 216 TCATGA T^CATGA
1259 TCATGA
ScaI 270 AGTACT AGT^ACT
1399 AGTACT
TfiI 1142 GAATC G^A[AT]TC
1248 GAATC
TspRI 974 CAGTG CA[GC]TG[ACGT][ACGT]^
1707 CAGTG
Van91I 387 CCACAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1036 CCAAAATATGG
XhoII 288 GGATCC [GA]^GATC[CT]
980 AGATCC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BbsI BbvI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp509I TthHB8I