Restriction map for
pFN21AE1877
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII AclI AcsI AcyI AflII
AgeI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbsI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MflI MluI MluNI MscI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 919 GGTACC G^GTACC
AccI 1174 GTCTAC GT^[AC][GT]AC
AccII 652 CGCG CG^CG
AccIII 368 TCCGGA T^CCGGA
AfaI 920 GTAC GT^AC
AflIII 872 ACGTGT A^C[GA][CT]GT
AhdI 604 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 177 GATCC GGATC(4/5)
AseI 228 ATTAAT AT^TAAT
AsnI 228 ATTAAT AT^TAAT
Asp700I 543 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 919 GGTACC G^GTACC
AspEI 604 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 565 GAGCTC G[AT]GC[AT]^C
BbrPI 388 CACGTG CAC^GTG
BbvCI 481 CCTCAGC CCTCAGC(-5/-2)
BcnI 222 CCGGG CC^[GC]GG
BfaI 32 CTAG C^TAG
BsaAI 388 CACGTG [CT]AC^GT[GA]
BsaWI 368 TCCGGA [AT]^CCGG[AT]
BseAI 368 TCCGGA T^CCGGA
BseRI 206 GAGGAG GAGGAG(10/8)
BsiHKAI 565 GAGCTC G[AT]GC[AT]^C
BspEI 368 TCCGGA T^CCGGA
BsrFI 343 GCCGGC [GA]^CCGG[CT]
BstUI 652 CGCG CG^CG
BstXI 10 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 343 GCCGGC [GA]^CCGG[CT]
DpnII 177 GATC ^GATC
DraIII 487 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1033 GACCTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 146 CGGCCA [CT]^GGCC[GA]
Eam1105I 604 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 51 GACGC GACGC(5/10)
KpnI 919 GGTACC GGTAC^C
MaeI 32 CTAG C^TAG
MboI 177 GATC ^GATC
MroI 368 TCCGGA T^CCGGA
MslI 964 CACAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 652 CGCG CG^CG
NaeI 343 GCCGGC GCC^GGC
NciI 222 CCGGG CC^[GC]GG
NdeII 177 GATC ^GATC
NgoMIV 343 GCCGGC G^CCGGC
PflMI 191 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 388 CACGTG CAC^GTG
PmlI 388 CACGTG CAC^GTG
PpuMI 707 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 228 ATTAAT AT^TAAT
PstI 97 CTGCAG CTGCA^G
RsaI 920 GTAC GT^AC
SacI 565 GAGCTC GAGCT^C
SapI 91 GAAGAGC GCTCTTC(1/4)
Sau3AI 177 GATC ^GATC
SmlI 668 CTCAAG C^T[CT][GA]AG
TfiI 105 GATTC G^A[AT]TC
Tsp509I 227 AATT ^AATT
Van91I 191 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1136 CCAAGGTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 543 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BpmI 137 CTGGAG CTGGAG(16/14)
404 CTGGAG
BsaI 739 GAGACC GGTCTC(1/5)
1041 GGTCTC
BsgI 154 CTGCAC GTGCAG(16/14)
159 CTGCAC
EarI 713 CTCTTC CTCTTC(1/4)
91 GAAGAG
FauI 595 CCCGC CCCGC(4/6)
650 CCCGC
Ksp632I 713 CTCTTC CTCTTC(1/4)
91 GAAGAG
MboII 91 GAAGA GAAGA(8/7)
714 TCTTC
MseI 229 TTAA T^TAA
398 TTAA
PvuII 134 CAGCTG CAG^CTG
847 CAGCTG
SexAI 797 ACCAGGT A^CC[AT]GGT
853 ACCTGGT
Tru9I 229 TTAA T^TAA
398 TTAA
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI ApaI AvaII BanI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MnlI MspA1I MspI
MvaI MwoI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI