Restriction map for
pFN21AE1766
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspHI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I CelII CpoI DraI
DraIII EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MluI MluNI MscI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StyI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 60 CGCG CG^CG
AccIII 506 TCCGGA T^CCGGA
AflIII 803 ACATGT A^C[GA][CT]GT
AlwNI 701 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 674 CCTCAGG CC^T[ACGT]AGG
ApaI 564 GGGCCC GGGCC^C
BamHI 630 GGATCC G^GATCC
BbsI 586 GAAGAC GAAGAC(2/6)
BglII 640 AGATCT A^GATCT
BpuAI 586 GAAGAC GAAGAC(2/6)
BsaI 392 GGTCTC GGTCTC(1/5)
BseAI 506 TCCGGA T^CCGGA
BseRI 382 GAGGAG GAGGAG(10/8)
BsmAI 393 GTCTC GTCTC(1/5)
BspDI 334 ATCGAT AT^CGAT
BspEI 506 TCCGGA T^CCGGA
BspLU11I 803 ACATGT A^CATGT
BspMI 46 ACCTGC ACCTGC(4/8)
BsrFI 36 GCCGGC [GA]^CCGG[CT]
BsrI 533 CCAGT ACTGG(1/-1)
BstEII 121 GGTGACC G^GT[ACGT]ACC
BstPI 121 GGTGACC G^GT[ACGT]ACC
BstUI 60 CGCG CG^CG
Bsu36I 674 CCTCAGG CC^T[ACGT]AGG
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
ClaI 334 ATCGAT AT^CGAT
DsaI 251 CCGTGG C^C[GA][CT]GG
EaeI 38 CGGCCA [CT]^GGCC[GA]
Eco81I 674 CCTCAGG CC^T[ACGT]AGG
EcoO65I 121 GGTGACC G^GT[ACGT]ACC
HgaI 58 GACGC GACGC(5/10)
MboII 586 GAAGA GAAGA(8/7)
MroI 506 TCCGGA T^CCGGA
MspA1I 164 CAGCTG C[AC]G^C[GT]G
MvnI 60 CGCG CG^CG
NaeI 36 GCCGGC GCC^GGC
NgoMIV 36 GCCGGC G^CCGGC
NspI 803 ACATGT [GA]CATG^[CT]
PstI 48 CTGCAG CTGCA^G
PvuII 164 CAGCTG CAG^CTG
SacI 719 GAGCTC GAGCT^C
SbfI 47 CCTGCAGG CCTGCA^GG
SfcI 48 CTGCAG C^T[GA][CT]AG
Sse8387I 47 CCTGCAGG CCTGCA^GG
StuI 678 AGGCCT AGG^CCT
TaqI 335 TCGA T^CGA
TthHB8I 335 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 16 GTAC GT^AC
807 GTAC
AspHI 656 GAGCAC G[AT]GC[AT]^C
719 GAGCTC
BanII 564 GGGCCC G[GA]GC[CT]^C
719 GAGCTC
BpmI 698 CTCCAG CTGGAG(16/14)
10 CTGGAG
BsaWI 200 TCCGGT [AT]^CCGG[AT]
506 TCCGGA
BsiHKAI 656 GAGCAC G[AT]GC[AT]^C
719 GAGCTC
BstF5I 216 GGATG GGATG(2/0)
324 GGATG
BstYI 630 GGATCC [GA]^GATC[CT]
640 AGATCT
DraII 132 GGGTCCC [GA]G^G[ACGT]CC[CT]
364 AGGACCT
DrdI 58 GACGCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
187 GACCTGTATGTC
EcoNI 607 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
670 CCTTCCTCAGG
EcoO109I 132 GGGTCCC [GA]G^G[ACGT]CC[CT]
364 AGGACCT
FauI 786 CCCGC CCCGC(4/6)
4 GCGGG
FokI 216 GGATG GGATG(9/13)
324 GGATG
HaeII 33 AGCGCC [GA]GCGC^[CT]
703 GGCGCT
HinfI 410 GAATC G^A[ACGT]TC
510 GATTC
MflI 630 GGATCC [GA]^GATC[CT]
640 AGATCT
PpuMI 132 GGGTCCC [GA]G^G[AT]CC[CT]
364 AGGACCT
RsaI 16 GTAC GT^AC
807 GTAC
SfaNI 337 GATGC GCATC(5/9)
25 GCATC
TfiI 410 GAATC G^A[AT]TC
510 GATTC
TspRI 378 CAGTG CA[GC]TG[ACGT][ACGT]^
433 CAGTG
XhoII 630 GGATCC [GA]^GATC[CT]
640 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TseI Tsp45I