Restriction map for
pFN21AE6460
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspHI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI MamI MboI
MfeI MflI MluI MroI MseI
MunI NarI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI Sau3AI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
TthHB8I XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 113 GGTACC G^GTACC
Aor51HI 1329 AGCGCT AGC^GCT
Asp718I 113 GGTACC G^GTACC
AspI 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 103 CCCGAG C^[CT]CG[GA]G
BanI 113 GGTACC G^G[CT][GA]CC
BfaI 862 CTAG C^TAG
BsaWI 481 TCCGGT [AT]^CCGG[AT]
BsoBI 103 CCCGAG C^[CT]CG[GA]G
BsrFI 392 GCCGGC [GA]^CCGG[CT]
Cfr10I 392 GCCGGC [GA]^CCGG[CT]
Eco47III 1329 AGCGCT AGC^GCT
EcoNI 1161 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 513 GATATC GAT^ATC
EcoT14I 1136 CCATGG C^C[AT][AT]GG
HinfI 437 GACTC G^A[ACGT]TC
KpnI 113 GGTACC GGTAC^C
KspI 1376 CCGCGG CCGC^GG
MaeI 862 CTAG C^TAG
NaeI 392 GCCGGC GCC^GGC
NcoI 1136 CCATGG C^CATGG
NgoMIV 392 GCCGGC G^CCGGC
NspI 947 GCATGC [GA]CATG^[CT]
PflFI 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 734 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 437 GACTC GAGTC(4/5)
PstI 824 CTGCAG CTGCA^G
SacII 1376 CCGCGG CCGC^GG
SapI 1365 GCTCTTC GCTCTTC(1/4)
SbfI 823 CCTGCAGG CCTGCA^GG
SexAI 773 ACCAGGT A^CC[AT]GGT
SfcI 824 CTGCAG C^T[GA][CT]AG
SphI 947 GCATGC GCATG^C
Sse8387I 823 CCTGCAGG CCTGCA^GG
StyI 1136 CCATGG C^C[AT][AT]GG
Tth111I 772 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 734 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 861 TCTAGA T^CTAGA
XcmI 416 CCACCTTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 735 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1218 CAGCGGCTG
ApaI 160 GGGCCC GGGCC^C
819 GGGCCC
BcnI 598 CCCGG CC^[GC]GG
888 CCCGG
BglI 457 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
821 GCCCTGCAGGC
BpmI 721 CTCCAG CTGGAG(16/14)
625 CTGGAG
BsmFI 1108 GGGAC GGGAC(10/14)
618 GTCCC
BspHI 386 TCATGA T^CATGA
686 TCATGA
BsrI 1175 ACTGG ACTGG(1/-1)
991 CCAGT
BstF5I 141 CATCC GGATG(2/0)
792 CATCC
FokI 141 CATCC GGATG(9/13)
792 CATCC
HaeII 1279 AGCGCC [GA]GCGC^[CT]
1329 AGCGCT
MaeII 1235 ACGT A^CGT
1355 ACGT
NciI 598 CCCGG CC^[GC]GG
888 CCCGG
PpuMI 1091 GGGTCCT [GA]G^G[AT]CC[CT]
1108 GGGACCT
RcaI 386 TCATGA T^CATGA
686 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BalI BanII BbsI BbvCI BbvI
BmyI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI Bsp1286I
BspMI BssKI BssSI BstEII BstNI
BstPI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HpaII HphI
ItaI Ksp632I MaeIII MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NlaIII NlaIV
PvuII RsaI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI