Restriction map for
pFN21AE5059
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HinfI HpaI HphI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluNI MroI MscI
MseI MslI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 174 GGACC G^G[AT]CC
BcgI 737 GCAAGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 190 CTGGAG CTGGAG(16/14)
BseRI 235 GAGGAG GAGGAG(10/8)
BsgI 48 GTGCAG GTGCAG(16/14)
BsmBI 398 GAGACG CGTCTC(1/5)
BsrBI 753 CCGCTC CCGCTC(-3/-3)
DdeI 508 CTGAG C^T[ACGT]AG
DpnII 27 GATC ^GATC
DraII 114 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 320 CCATGG C^C[GA][CT]GG
EcoO109I 114 GGGGCCT [GA]G^G[ACGT]CC[CT]
MboI 27 GATC ^GATC
MluI 464 ACGCGT A^CGCGT
NcoI 320 CCATGG C^CATGG
NdeII 27 GATC ^GATC
PflMI 75 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 679 CTGCAG CTGCA^G
PvuII 547 CAGCTG CAG^CTG
Sau3AI 27 GATC ^GATC
SfaNI 169 GATGC GCATC(5/9)
SfcI 679 CTGCAG C^T[GA][CT]AG
SmlI 835 CTCAAG C^T[CT][GA]AG
Van91I 75 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 464 ACGCGT A^C[GA][CT]GT
584 ACGTGT
BanII 208 GAGCCC G[GA]GC[CT]^C
390 GGGCTC
BglI 874 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
907 GCCGGCCCGGC
BsaAI 94 TACGTG [CT]AC^GT[GA]
583 TACGTG
BsmAI 85 GAGAC GTCTC(1/5)
398 GAGAC
BsrI 45 CCAGT ACTGG(1/-1)
813 CCAGT
BstAPI 144 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
621 GCACAACCTGC
EagI 203 CGGCCG C^GGCCG
881 CGGCCG
EclXI 203 CGGCCG C^GGCCG
881 CGGCCG
Eco52I 203 CGGCCG C^GGCCG
881 CGGCCG
MaeIII 304 GTGAC ^GT[ACGT]AC
686 GTCAC
MboII 24 GAAGA GAAGA(8/7)
332 TCTTC
NspI 689 ACATGC [GA]CATG^[CT]
773 ACATGC
SgrAI 221 CGCCGGCG C[GA]^CCGG[CT]G
702 CACCGGCG
SmaI 110 CCCGGG CCC^GGG
121 CCCGGG
TaqI 265 TCGA T^CGA
502 TCGA
Tsp45I 304 GTGAC ^GT[GC]AC
686 GTCAC
TthHB8I 265 TCGA T^CGA
502 TCGA
XmaCI 110 CCCGGG C^CCGGG
121 CCCGGG
XmaI 110 CCCGGG C^CCGGG
121 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrFI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I EaeI
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NgoMIV
NlaIII NlaIV RsaI Sau96I ScrFI
SfoI StuI StyI TseI TspRI