Restriction map for
pFN21AB9650
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NheI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 324 GTAC GT^AC
Asp700I 132 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 657 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 85 GAGCCC G[GA]GC[CT]^C
BbsI 499 GAAGAC GAAGAC(2/6)
BbvCI 709 GCTGAGG CCTCAGC(-5/-2)
BfaI 917 CTAG C^TAG
BglII 206 AGATCT A^GATCT
BpuAI 499 GAAGAC GAAGAC(2/6)
BsaI 538 GAGACC GGTCTC(1/5)
BsmBI 727 GAGACG CGTCTC(1/5)
BsmI 136 GCATTC GAATGC(1/-1)
BspMI 824 ACCTGC ACCTGC(4/8)
BstAPI 234 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 882 GGTTACC G^GT[ACGT]ACC
BstF5I 563 GGATG GGATG(2/0)
BstPI 882 GGTTACC G^GT[ACGT]ACC
BstXI 212 CCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 710 CTGAG C^T[ACGT]AG
DrdI 729 GACGGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 432 CCGCGG C^C[GA][CT]GG
EcoO65I 882 GGTTACC G^GT[ACGT]ACC
FokI 563 GGATG GGATG(9/13)
HgaI 736 GCGTC GACGC(5/10)
KspI 432 CCGCGG CCGC^GG
MaeI 917 CTAG C^TAG
MaeII 844 ACGT A^CGT
MaeIII 883 GTTAC ^GT[ACGT]AC
NotI 631 GCGGCCGC GC^GGCCGC
NruI 813 TCGCGA TCG^CGA
NspI 191 ACATGC [GA]CATG^[CT]
PflFI 657 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 54 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 324 GTAC GT^AC
SacII 432 CCGCGG CCGC^GG
SexAI 929 ACCTGGT A^CC[AT]GGT
SfaNI 920 GCATC GCATC(5/9)
TspRI 181 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 657 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 132 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 258 GATCC GGATC(4/5)
810 GGATC
AlwNI 235 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
704 CAGCGGCTG
BglI 431 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
782 GCCCGGCCGGC
BmyI 85 GAGCCC G[AGT]GC[ACT]^C
840 GGGCAC
BsgI 870 CTGCAC GTGCAG(16/14)
4 GTGCAG
BsmAI 538 GAGAC GTCTC(1/5)
727 GAGAC
Bsp1286I 85 GAGCCC G[AGT]GC[ACT]^C
840 GGGCAC
BsrI 905 ACTGG ACTGG(1/-1)
180 CCAGT
BssHII 79 GCGCGC G^CGCGC
603 GCGCGC
BstYI 206 AGATCT [GA]^GATC[CT]
257 AGATCC
DraII 54 GGGACCC [GA]G^G[ACGT]CC[CT]
456 AGGGCCT
EarI 685 GAAGAG CTCTTC(1/4)
721 GAAGAG
EcoO109I 54 GGGACCC [GA]G^G[ACGT]CC[CT]
456 AGGGCCT
EcoT14I 359 CCAAGG C^C[AT][AT]GG
609 CCAAGG
FseI 62 GGCCGGCC GGCCGG^CC
786 GGCCGGCC
HinfI 247 GACTC G^A[ACGT]TC
748 GAGTC
HphI 718 GGTGA GGTGA(8/7)
938 TCACC
Ksp632I 685 GAAGAG CTCTTC(1/4)
721 GAAGAG
MflI 206 AGATCT [GA]^GATC[CT]
257 AGATCC
PleI 247 GACTC GAGTC(4/5)
748 GAGTC
PvuII 235 CAGCTG CAG^CTG
421 CAGCTG
SfiI 430 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
781 GGCCCGGCCGGCC
SmlI 292 CTCAAG C^T[CT][GA]AG
406 CTCAAG
StyI 359 CCAAGG C^C[AT][AT]GG
609 CCAAGG
TaqI 536 TCGA T^CGA
854 TCGA
TthHB8I 536 TCGA T^CGA
854 TCGA
XhoII 206 AGATCT [GA]^GATC[CT]
257 AGATCC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BbeI BbvI BcnI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI BsrBI BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfcI SfoI
SmaI TseI XmaCI XmaI