##################################
# NO CUT ENZYMES (  215 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
KspI        MaeI        MamI        MboI        MflI        
MluI        MluNI       MnlI        MroI        MscI        
MseI        MslI        MspI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TfiI        Tru9I       TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI          155  GCGG                 CCGC(-3/-1)
AluI          183  AGCT                 AG^CT
BbsI          297  GTCTTC               GAAGAC(2/6)
BpuAI         297  GTCTTC               GAAGAC(2/6)
BsaI           51  GGTCTC               GGTCTC(1/5)
BsmAI          52  GTCTC                GTCTC(1/5)
BsrDI         148  GCAATG               GCAATG(2/0)
BsrI          328  ACTGG                ACTGG(1/-1)
BssKI          39  CCTGG                ^CC[ACGT]GG
BstNI          39  CCTGG                CC^[AT]GG
Cfr13I         42  GGCCC                G^G[ACGT]CC
DdeI           54  CTCAG                C^T[ACGT]AG
EarI          121  GAAGAG               CTCTTC(1/4)
EcoRII         39  CCTGG                ^CC[AT]GG
EcoT22I       127  ATGCAT               ATGCA^T
Fnu4HI        155  GCGGC                GC^[ACGT]GC
HaeIII         42  GGCC                 GG^CC
HphI           17  TCACC                GGTGA(8/7)
ItaI          155  GCGGC                GC^[ACGT]GC
Ksp632I       121  GAAGAG               CTCTTC(1/4)
MaeIII        247  GTAAC                ^GT[ACGT]AC
MfeI           46  CAATTG               C^AATTG
MspA1I        182  CAGCTG               C[AC]G^C[GT]G
MunI           46  CAATTG               C^AATTG
MvaI           39  CCTGG                CC^[AT]GG
MwoI          253  GCAACAACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        130  CATG                 CATG^
NlaIV         306  GGCTCC               GG[ACGT]^[ACGT]CC
NsiI          127  ATGCAT               ATGCA^T
NspI          129  GCATGT               [GA]CATG^[CT]
PvuII         182  CAGCTG               CAG^CTG
Sau96I         42  GGCCC                G^G[ACGT]CC
ScrFI          39  CCTGG                CC^[ACGT]GG
SfaNI         126  GATGC                GCATC(5/9)
TspRI         327  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AcsI           95  GAATTT               [GA]^AATT[CT]
              236  GAATTT              
ApoI           95  GAATTT               [GA]^AATT[CT]
              236  GAATTT              
BsmFI         167  GGGAC                GGGAC(10/14)
                6  GTCCC               
MaeII          89  ACGT                 A^CGT
              192  ACGT                
TaqI          292  TCGA                 T^CGA
              301  TCGA                
TthHB8I       292  TCGA                 T^CGA
              301  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

MboII       Tsp509I