Restriction map for
pFN21AE1664
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 78 GGCGTC G[GA]^CG[CT]C
AflIII 136 ACGTGT A^C[GA][CT]GT
BamHI 98 GGATCC G^GATCC
BanI 55 GGCACC G^G[CT][GA]CC
BanII 412 GAGCCC G[GA]GC[CT]^C
BclI 2 TGATCA T^GATCA
BmyI 412 GAGCCC G[AGT]GC[ACT]^C
BsaHI 78 GGCGTC G[GA]^CG[CT]C
BsgI 490 GTGCAG GTGCAG(16/14)
BsmAI 286 GAGAC GTCTC(1/5)
Bsp1286I 412 GAGCCC G[AGT]GC[ACT]^C
BspMI 125 ACCTGC ACCTGC(4/8)
BsrFI 467 ACCGGC [GA]^CCGG[CT]
BssKI 249 CCAGG ^CC[ACGT]GG
BstEII 253 GGTTACC G^GT[ACGT]ACC
BstF5I 339 CATCC GGATG(2/0)
BstNI 249 CCAGG CC^[AT]GG
BstPI 253 GGTTACC G^GT[ACGT]ACC
Cfr10I 467 ACCGGC [GA]^CCGG[CT]
EarI 403 CTCTTC CTCTTC(1/4)
EcoNI 342 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 253 GGTTACC G^GT[ACGT]ACC
EcoRII 249 CCAGG ^CC[AT]GG
EcoRV 142 GATATC GAT^ATC
FbaI 2 TGATCA T^GATCA
FokI 339 CATCC GGATG(9/13)
HapII 468 CCGG C^CGG
HgaI 79 GCGTC GACGC(5/10)
Hin1I 78 GGCGTC G[GA]^CG[CT]C
HinfI 43 GAGTC G^A[ACGT]TC
HpaII 468 CCGG C^CGG
Ksp632I 403 CTCTTC CTCTTC(1/4)
MaeIII 254 GTTAC ^GT[ACGT]AC
MseI 239 TTAA T^TAA
MspA1I 91 CAGCTG C[AC]G^C[GT]G
MspI 468 CCGG C^CGG
MvaI 249 CCAGG CC^[AT]GG
PaeR7I 316 CTCGAG C^TCGAG
PleI 43 GAGTC GAGTC(4/5)
PshAI 288 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 91 CAGCTG CAG^CTG
ScrFI 249 CCAGG CC^[ACGT]GG
SmlI 316 CTCGAG C^T[CT][GA]AG
TaqI 317 TCGA T^CGA
Tru9I 239 TTAA T^TAA
Tsp509I 384 AATT ^AATT
TspRI 431 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 317 TCGA T^CGA
XhoI 316 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Aor51HI 494 AGCGCT AGC^GCT
536 AGCGCT
AvaI 316 CTCGAG C^[CT]CG[GA]G
421 CCCGAG
AvaII 426 GGACC G^G[AT]CC
453 GGTCC
BsiYI 196 CCTGATGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
342 CCTCAGAGAGG
BslI 196 CCTGATGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
342 CCTCAGAGAGG
BsoBI 316 CTCGAG C^[CT]CG[GA]G
421 CCCGAG
BsrI 353 ACTGG ACTGG(1/-1)
361 CCAGT
BstAPI 323 GCAACGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
483 GCAGAACGTGC
BstYI 98 GGATCC [GA]^GATC[CT]
156 AGATCC
Cac8I 49 GCGGGC GC[ACGT]^[ACGT]GC
392 GCCTGC
DdeI 153 CTCAG C^T[ACGT]AG
343 CTCAG
DraII 425 AGGACCC [GA]G^G[ACGT]CC[CT]
452 AGGTCCT
Eco47III 494 AGCGCT AGC^GCT
536 AGCGCT
EcoO109I 425 AGGACCC [GA]G^G[ACGT]CC[CT]
452 AGGTCCT
EcoT14I 59 CCAAGG C^C[AT][AT]GG
272 CCAAGG
HaeII 494 AGCGCT [GA]GCGC^[CT]
536 AGCGCT
HphI 276 GGTGA GGTGA(8/7)
300 TCACC
MboII 30 GAAGA GAAGA(8/7)
404 TCTTC
MflI 98 GGATCC [GA]^GATC[CT]
156 AGATCC
NlaIII 262 CATG CATG^
501 CATG
PpuMI 425 AGGACCC [GA]G^G[AT]CC[CT]
452 AGGTCCT
PstI 394 CTGCAG CTGCA^G
457 CTGCAG
SfcI 394 CTGCAG C^T[GA][CT]AG
457 CTGCAG
StyI 59 CCAAGG C^C[AT][AT]GG
272 CCAAGG
XhoII 98 GGATCC [GA]^GATC[CT]
156 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BseRI CfoI Cfr13I DpnII FauI
Fnu4HI HaeIII HhaI HinP1I ItaI
MaeII MboI MnlI MwoI NdeII
NlaIV Sau3AI Sau96I TseI